Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil
Emergence of NDM-1-producing Enterobacteriaceae in Porto Alegre, Brazil
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DOI：
10.1016/j.ijid.2014.01.005
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发表时间：
2014-08-01
影响因子：
8.4
通讯作者：
Zavascki, Alexandre P.
中科院分区：
文献类型：
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作者：
Rozales, Francieli P.;Ribeiro, Vanessa B.;Zavascki, Alexandre P.
目的：了解新德里产金属β-内酰胺酶1(NDM-1)肠杆菌科细菌在巴西的出现情况。方法：自2013年4月至10月，在巴西阿雷格里港17家医院对碳青霉烯类抗生素敏感性降低的肠杆菌科细菌中检测BLA(NDM-1)。用实时定量聚合酶链式反应检测碳青霉烯酶基因的存在，并对其进行测序。用脉冲场凝胶电泳法(PFGE)分析克隆亲缘关系。结果：共鉴定出1134株细菌。在11株(0.97%)菌株中检出blaNDM-1：9株阴沟肠杆菌复合体(8株来自两家不同医院的单一克隆，另一株来自第三家医院)和2株摩根摩根菌(属于一家医院的单一克隆)。大多数菌株对碳青霉烯类呈高度耐药。结论：产NDM-1肠杆菌科细菌在首次发现NDM-1肠杆菌科细菌的巴西城市的医院中迅速出现。NDM-1在巴西的出现引起了人们的极大关注，因为它对该国肠杆菌科的抗菌治疗构成了严重威胁。(C)2014年提交人。爱思唯尔有限公司代表国际传染病学会出版。
Objectives: To evaluate the emergence of New Delhi metallo-beta-lactamase 1 (NDM-1)-producing Enterobacteriaceae isolates in Brazil.Methods: From April to October 2013, following the detection of the first NDM-1-producing isolate, a surveillance study was performed for the detection of bla(NDM-1) among Enterobacteriaceae isolates with reduced susceptibility to carbapenems in 17 hospitals of Porto Alegre, Brazil. Real-time PCR was used to determine the presence of carbapenemase genes, which were further sequenced. Clonal relatedness was assessed by pulsed-field gel electrophoresis (PFGE).Results: A total of 1134 isolates were evaluated. blaNDM-1 was detected in 11 (0.97%) isolates: nine Enterobacter cloacae complex (eight belonging to a single clone recovered from two distinct hospitals and the other strain from a third hospital) and two Morganella morganii (belonging to a single clone recovered from one hospital). Most isolates presented high-level resistance to carbapenems.Conclusions: NDM-1-producing Enterobacteriaceae have emerged rapidly in the hospitals of the Brazilian city where they were first detected. The emergence of NDM-1 in Brazil is of great concern, since it is a severe threat to antimicrobial therapy against Enterobacteriaceae in this country. (C) 2014 The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.