Relation between energy production and adenine nucleotide metabolism in human blood platelets.
Relation between energy production and adenine nucleotide metabolism in human blood platelets.
复制标题
人体血小板能量产生与腺嘌呤核苷酸代谢之间的关系。
DOI：
10.1016/0005-2728(80)90150-4
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
G. Gorter
中科院分区：
文献类型：
--
作者：
J. Akkerman;G. Gorter
在人血小板中研究了ATP产生和腺嘌呤核苷酸代谢之间的关系，该血小板通过在无葡萄糖、含CN−的培养基中孵育并随后与不同量的葡萄糖孵育而饥饿。在缺乏线粒体能量产生（被CN-阻断）和糖原分解（糖原在饥饿期间几乎完全消耗）的情况下，乳酸的产生随着葡萄糖量的增加而成比例地增加。产生的ATP几乎完全消耗在细胞中的各种ATP消耗过程中，除了一个固定的部分（约7%），这是保留用于恢复腺苷酸能量电荷。在加入葡萄糖后的前10分钟内，腺嘌呤核苷酸库保持恒定。此后，当糖酵解通量（以乳酸盐形成量测量）超过3.5μmol · min-1· 10 - 11个细胞时，次黄嘌呤-肌苷的再合成使池略有增加，然后稳定;在较低的通量下，池减少，代谢ATP和能荷下降到饥饿期间发现的值。在腺苷酸能量电荷上升和下降的时刻之间，保持约10分钟的时间，其中电荷恒定，ATP供给和需求达到平衡。这使得能够比较腺苷酸能荷和ATP再生速度。当电荷值在0.4 ~ 0.85之间，ATP再生率在0.6 ~ 3.5ATP当量之间时，获得了线性关系。· min−1· 10− 11个细胞。这些数据表明，在饥饿的血小板ATP再生速度和能量电荷是独立的，每个似乎是受胞外底物的可用性。
The relation between ATP production and adenine nucleotide metabolism was investigated in human platelets which were starved by incubation in glucose-free, CN−-containing medium and subsequently incubated with different amounts of glucose. In the absence of mitochondrial energy production (blocked by CN−) and glycogen catabolism (glycogen almost completely consumed during starvation), lactate production increased proportionally with increasing amounts of glucose. The generated ATP was almost completely consumed in the various ATP-consuming processes in the cell except for a fixed portion (about 7%) that was reserved for restoration of the adenylate energy charge. During the first 10 min after glucose addition, the adenine nucleotide pool remained constant. Thereafter, when the glycolytic flux, measured as lactate formation, was more than 3.5μmol · min−1· 10−11cells, the pool increased slightly by resynthesis from hypoxanthine-inosine and then stabilized; at a lower flux the pool decreased and metabolic ATP and energy charge declined to values found during starvation. Between moments of rising and falling adenylate energy charges, periods of about 10 min remained in which the charge was constant and ATP supply and demand had reached equilibrium. This enabled comparison between the adenylate energy charge and ATP regeneration velocity. A linear relation was obtained for charge values between 0.4 and 0.85 and ATP regeneration rates between 0.6 and 3.5 ATP equiv. · min−1· 10−11cells. These data indicate that in starved platelets ATP regeneration velocity and energy charge are independent and that each appears to be subject to the availability of extracellular substrate.