AN EFFICIENT AND EASY METHOD OF INFECTION OF MOSQUITO LARVAE FROM VIRUS-CONTAMINATED CELL-CULTURES
AN EFFICIENT AND EASY METHOD OF INFECTION OF MOSQUITO LARVAE FROM VIRUS-CONTAMINATED CELL-CULTURES
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DOI：
10.1016/0166-0934(94)90039-6
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发表时间：
1994-09-01
影响因子：
3.1
通讯作者：
CORNET, M
中科院分区：
文献类型：
--
作者：
BARREAU, C;JOUSSET, FX;CORNET, M
描述了一种用白纹伊蚊浓核病毒（AaPV）有效感染埃及伊蚊幼虫的新方法。它包括将一龄或三龄幼虫置于含有慢性感染蚊子细胞系的培养瓶中。在暴露于污染的培养物24或48小时后，幼虫通过进食感染的细胞获得病毒。利用这种技术，高达95%的第一龄Ae。发现埃及伊蚊幼虫被AaPV感染。
A new method for efficient infection of Aedes aegypti larvae by the Aedes albopictus densovirus, AaPV is described. It consists of placing first or third instar larvae in culture flasks containing a chronically infected mosquito cell line. After 24 or 48 h of exposure to the contaminated culture, the larvae acquired the virus by feeding on infected cells. Using this technique, up to 95% of first instar Ae. aegypti larvae were found infected by the AaPV.