A survey of Fusobacterium nucleatum genes modulated by host cell infection
A survey of Fusobacterium nucleatum genes modulated by host cell infection
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DOI：
10.1099/mgen.0.000300
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发表时间：
2020-02-01
影响因子：
3.9
通讯作者：
Allen-Vercoe, Emma
中科院分区：
文献类型：
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作者：
Cochrane, Kyla;Robinson, Avery, V;Allen-Vercoe, Emma
在这里，我们报告了全面的转录组配置文件从具核梭杆菌的条件下，模拟细菌感染的第一阶段，在高度分化的腺癌上皮细胞系。我们的体外腺癌转录组学方法使我们能够在真实的时间内测量细菌毒力和应答因子的表达动态和调节，并且是阐明F.结直肠癌（CRC）进展中的核感染。我们的数据表明：（i）感染改变了代谢和功能途径在F。（ii）感染还刺激帮助诱导和促进宿主中缺氧和炎症微环境所需的基因的表达;和（iii）F.通过血行感染途径发生核侵入。我们的研究从F.在入侵过程中被激活，并可能有助于确定该物种如何入侵和促进人类胃肠道内的疾病。这些侵袭特异性基因可用作宿主中CRC进展的生物标志物，并且还可有助于开发新的诊断工具和治疗（如疫苗或小分子药物靶标），其将能够对抗宿主中的感染和炎症，同时规避F.核质耐受
Here, we report comprehensive transcriptomic profiles from Fusobacterium nucleatum under conditions that mimic the first stages of bacterial infection in a highly differentiated adenocarcinoma epithelial cell line. Our transcriptomic in vitro adenocarcinoma approach allows us to measure the expression dynamics and regulation of bacterial virulence and response factors in real time, and is a novel strategy for clarifying the role of F. nucleatum infection in colorectal cancer (CRC) progression. Our data show that: (i) infection alters metabolic and functional pathways in F. nucleatum, allowing the bacterium to adapt to the host-imposed milieu; (ii) infection also stimulates the expression of genes required to help induce and promote a hypoxic and inflammatory microenvironment in the host; and (iii) F. nucleatum invasion occurs by a haematogenous route of infection. Our study identifies novel gene targets from F. nucleatum that are activated during invasion and which may aid in determining how this species invades and promotes disease within the human gastrointestinal tract. These invasion-specific genes may be useful as biomarkers for CRC progression in a host and could also assist in the development of new diagnostic tools and treatments (such as vaccines or small molecule drug targets), which will be able to combat infection and inflammation in the host while circumventing the potential problem of F. nucleatum tolerization.