Cytokeratin Expression at Different Stages in Sweat Gland Development of C57BL/6J Mice
Cytokeratin Expression at Different Stages in Sweat Gland Development of C57BL/6J Mice
复制标题
C57BL/6J小鼠汗腺发育不同阶段细胞角蛋白的表达
DOI：
10.1177/1534734615611563
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发表时间：
2015-12-01
影响因子：
1.7
通讯作者：
Fu, Xiaobing
中科院分区：
文献类型：
--
作者：
Xie, Jiangfan;Yao, Bin;Fu, Xiaobing
汗腺在创伤后表皮再上皮化中的作用已被证实。然而，汗腺的再生潜力仍然被低估，由于缺乏有用的标记物，用于分析从上皮细胞发育的外分泌汗腺的确定和分化过程。虽然目前的知识角蛋白的表达在大多数不同的起源已被描述，它仍然是广泛共享的，而不是统一的外分泌汗腺的C57 BL/6 J小鼠，通常用作动物模型的汗腺和伤口愈合的研究，无论是在分子和细胞水平。为了回答这个问题，我们研究了细胞角蛋白表达模式在胚胎，新生儿，青少年和年轻成人阶段（E12.5，E17.5，P0.5，P5和P28）的变化。在这篇文章中，我们证明，小鼠汗腺祖细胞的形态类似于表皮干细胞出生前（E12.5和E17.5），在出生后的阶段，导管逐渐形成，卷曲成球状。K8和K19在所有时间都在外泌汗腺细胞中表达，并且在基因和蛋白水平上在出生后高度表达。组织学结果显示K8和K19阳性细胞定位于腺体的分泌部。同时，K14在体内和体外均在E12.5强表达，而在其他阶段弱表达。此外，K10在出生前很少被检测到，但出生后在体内和体外仅在蛋白水平上呈阳性表达。这些数据表明，在小鼠汗腺发育的不同阶段，主要的细胞角蛋白的表达模式，并可能提供一个有效的工具，汗腺研究和令人兴奋的潜力，为伤口愈合的靶向治疗。
Sweat glands exhibit a documented role in epidermal reepithelialization after wounding. However, the regenerative potential of sweat glands has remained underappreciated due to the absence of useful markers for the analysis of determination and differentiation processes in the developing eccrine sweat gland from epithelium. Although the current knowledge of keratin expression in most of the different origins has been described, it remains widely shared and not unified in eccrine sweat glands of C57BL/6J mice that are commonly used as animal models for sweat gland and wound healing studies, both at the molecular and cellular levels. Aiming to answer this question, we have investigated the changes in cytokeratin expression patterns during the embryonic, neonatal, juvenile, and young adult stages (E12.5, E17.5, P0.5, P5, and P28). In this article, we demonstrate that the morphology of murine sweat gland progenitor cells are similar to epidermal stem cells before birth (E12.5 and E17.5); at postnatal stages, the duct formed gradually and curled to glob. K8 and K19 were expressed in the eccrine sweat gland cells at all times and highly expressed after birth at both gene and protein levels. Also, histological results revealed K8 and K19 positive cells localized in the secretary portion of glands. Meanwhile, K14 strongly expressed both in vivo and in vitro at E12.5, while it weakly expressed at other stages. Moreover, K10 was rarely detected before birth, but it expressed positively in vivo and in vitro only at the protein level after birth. These data indicate the pattern of main cytokeratin expression at different stages during murine sweat gland development and might provide an efficient tool for sweat gland research and exciting potential for developing targeted therapies for wound healing.