Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2.
Maturation of neurotransmission in the developing rat cochlea: immunohistochemical evidence from differential expression of synaptophysin and synaptobrevin 2.
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发育中的大鼠耳蜗神经传递的成熟：突触素和突触短蛋白 2 差异表达的免疫组织化学证据
DOI：
10.4081/ejh.2011.e2
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发表时间：
2011-01-18
期刊：
影响因子：
--
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
He S;Yang J
突触泡蛋白和突触泡蛋白2与突触囊泡的包装、储存和递质释放密切相关，在大鼠耳蜗发育中起重要作用。我们研究了突触体素和小突触素2在发育中的Sprague-Dawley大鼠耳蜗中的差异表达，并探讨了它们的表达与听觉发育之间的关系。突触泡蛋白和小突触泡蛋白2在生后1d（P1）和P5的Kolliker和Corti器及P10的耳蜗顶转处未见表达。P14、P28在耳蜗外螺旋束（OSB）、内螺旋束（ISB）、Deiters细胞内侧壁表达，P10在Corti器中、底回表达。在P14和P28大鼠的Corti器内毛细胞（IHC）的顶部表达Synaptobrevin 2。所有螺旋神经节神经元（SGN）染色在所有年龄检查。突触泡蛋白和小突触泡蛋白2在耳蜗中的定位与神经纤维的分布和神经活动（突触囊泡的对接和释放）密切相关。突触泡蛋白和突触泡蛋白2在大鼠耳蜗发育过程中呈动态表达。它们在发育过程中的表达差异有利于神经末梢与靶细胞之间构建的构型过程。在听觉系统发育过程中，它对听觉信息正确编码的形成也起着关键作用。
Synaptophysin and synaptobrevin 2 associate closely with packaging and storage of synaptic vesicles and transmitter release, and both play important roles in the development of rat cochlea. We examined the differential expression of synaptophysin and synaptobrevin 2 in the developing Sprague-Dawley rat cochlea, and investigated the relationship between their expression and auditory development. The expression of synaptophysin and synaptobrevin 2 was not observed in Kolliker's and Corti's organ at postnatal 1 day (P1) and P5, and the top turn of the cochlea at P10. Expression was detected in the outer spiral bundle (OSB), the inner spiral bundle (ISB), and the medial wall of the Deiters' cell of the cochlea at P14, and P28, and in the middle or the basal turn of Corti's organ at P10. Synaptobrevin 2 was expressed in the top of the inner hair cells (IHCs) in Corti's organ of both P14 and P28 rats. All spiral ganglion neurons (SGNs) were stained at all ages examined. The localization of synaptophysin and synaptobrevin 2 in the cochlea was closely associated with the distribution of nerve fibers and neural activity (the docking and release of synaptic vesicles). Synaptophysin and synaptobrevin 2 were expressed in a dynamic manner during the development of rat cochlea. Their expression differences during the development were in favor of the configuration course constructed between nerve endings and target cells. It also played a key role in the formation of the correct coding of auditory information during auditory system development.
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影响因子：
3.3
作者：
Nemzou, R. M.;Bulankina, A. V.;Moser, T.
通讯作者：
Moser, T.
影响因子：
3.1
作者：
Layton, MG;Robertson, D;Yates, GK
通讯作者：
Yates, GK
影响因子：
2.3
作者：
Elfving, Betina;Bonefeld, Birgit Egeskov;Wegener, Gregers
通讯作者：
Wegener, Gregers
影响因子：
4.1
作者：
Bonanomi, Dario;Rusconi, Laura;Valtorta, Flavia
通讯作者：
Valtorta, Flavia
影响因子：
2.8
作者：
Liu, Wei;Bostrom, Marja;Rask-Andersen, Helge
通讯作者：
Rask-Andersen, Helge