Pantoea agglomerans YS19 poly(A) polymerase i gene possesses the indole sensing sequence in the promoter region
Pantoea agglomerans YS19 poly(A) polymerase i gene possesses the indole sensing sequence in the promoter region
复制标题
成团泛菌 YS19 Poly(A) 聚合酶 i 基因在启动子区域具有吲哚感应序列
DOI：
10.1093/femsle/fnz166
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发表时间：
2019
影响因子：
2.1
通讯作者：
Yongjun Feng
中科院分区：
文献类型：
--
作者：
Jing Zheng;Qi Liu;Yifan Xia;Lijuan Bai;Yongjun Feng
从水稻中分离到的Pantoea agregansYS19是一种优势重氮营养内生菌，对其寄主植物具有多种促进生长作用。吲哚被证实能引起细菌的许多生理生化特性的变化。虽然YS19不能产生吲哚，但它能感知环境中的吲哚，并受吲哚的调控。在此，我们以gfpA为报告基因，构建了一系列含有与gfp融合的聚(A)聚合酶I基因(PcnB)启动子区域的重组载体，并用流式细胞仪比较了不同浓度外源吲哚的绿色荧光强度。在本研究中，我们证实了外源吲哚通过其启动子显著抑制了pcnB基因的表达，在pcnB基因启动子区域对吲哚敏感的调控序列(in-pcnB)介于−129和−88之间，in-pcnB在同一属中分布广泛且严格保守。这些结果提示了in-pcnBinP的新作用。AgregansYS19，显示了它与吲哚调节途径的特殊关系。
Pantoea agglomeransYS19 is a predominant diazotrophic endophyte with multiple growth-promoting effects on its host plant that was isolated from rice. Indole is confirmed to induce many changes of physiological and biochemical characteristics in bacteria. Although YS19 cannot produce indole, it can sense indole in the environment and be regulated by indole. Here, usinggfpas a reporter gene, we constructed a series of recombinant plasmids containing the promoter region of the poly(A) polymerase I gene (pcnB) fused withgfp, and compared the green fluorescence intensity at different concentrations of exogenous indole by a flow cytometer. In this research, we confirmed that exogenous indole significantly inhibited the expression ofpcnBby its promoter; the regulation sequence sensitive to indole in the promoter region of thepcnBgene (In-pcnB) was between −129 and −88 bp.In-pcnBis widely distributed and strictly conserved in the same genus. These results suggest novel roles ofIn-pcnBinP. agglomeransYS19, showing its special relation to the indole regulatory pathway.