Productive human immunodeficiency virus-1 infection of epithelial cell lines of salivary gland origin.
Productive human immunodeficiency virus-1 infection of epithelial cell lines of salivary gland origin.
复制标题
唾液腺来源的上皮细胞系的生产性人类免疫缺陷病毒-1 感染。
DOI：
10.1034/j.1399-302x.2000.150203.x
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发表时间：
2000
影响因子：
--
通讯作者：
Jackson,S
中科院分区：
文献类型：
--
作者：
Han,Y;Ventura,CL;Black,KP;CumminsJr,JE;Hall,SD;Jackson,S
为了确定口腔来源的上皮细胞是否可能感染人类免疫缺陷病毒（HIV-1），进行了一项研究以确定唾液腺上皮细胞系（HSY和HSG）感染的易感性。由于口腔生殖器传播的潜力，还研究了子宫内膜细胞系 HEC-1。上皮细胞单层被无细胞 HTLVIIIB 或初级 HIV-1 分离株感染。多项证据表明，用 HIV-1 接种这些细胞系会导致有效感染：1）p24 抗原存在于上清液中，其水平在第 3-4 天达到峰值； 2）通过聚合酶链式反应在细胞中发现原病毒； 3) 与 T 淋巴母细胞系 CEM-NKr 共培养证实，上清液中存在的病毒体具有传染性。经过一段时间的病毒产生后，HIV-1 进入了长达 10 周的潜伏期。所有上皮细胞系半乳糖神经酰胺 (GalC) 和 CXCR4 均呈阳性。 HSY 表面 CD4 呈弱阳性，并且也表达 CD4 和 CCR5 的 mRNA，HEC-1 也是如此。阻断研究表明，抗GalC（而非抗CD4）显着减少了生产性感染，并且调节正常T细胞表达和分泌的激活（RANTES）而非基质细胞衍生因子（SDF-1）可以部分阻断M-tropic初级分离株的感染。这些结果表明，口腔和生殖道的上皮细胞可能是 HIV-1 的目标，并可能充当全身感染的介质。
To ascertain whether epithelial cells of oral cavity origin may be infected with human immunodeficiency virus (HIV‐1), a study to determine susceptibility to infection of salivary gland epithelial cell lines (HSY and HSG) was undertaken. Because of the potential for oral‐genital transmission, an endometrial cell line, HEC‐1, was also studied. Epithelial cell monolayers were infected with cell‐free HTLVIIIBor a primary HIV‐1 isolate. Several lines of evidence indicated that inoculation of these cell lines with HIV‐1 led to productive infection: 1) p24 antigen was present in supernatants, with levels peaking on days 3–4; 2) provirus was found in cells by polymerase chain reaction; 3) virions present in supernatants were infectious as confirmed by coculture with the T‐lympho‐blastoid line CEM‐NKr. Following a period of virus production, HIV‐1 entered a latency phase over 10 weeks. All epithelial cell lines were positive for galactosylceramide (GalC) and CXCR4. HSY was weakly positive for surface CD4, and also expressed mRNA for CD4 and CCR5, as did HEC‐1. Blocking studies indicated that anti‐GalC, but not anti‐CD4, significantly reduced productive infection, and that regulated on activation normal T cell expressed and secreted (RANTES) but not stromal cell–derived factor (SDF‐1) could partially block infection of the M‐tropic primary isolate. These results suggest that epithelial cells in the oral cavity and the genital tract might be targets of HIV‐1 and potentially serve as a mediator of systemic infection.