Activation of cytokine genes in T cells during primary and secondary murine influenza pneumonia.
Activation of cytokine genes in T cells during primary and secondary murine influenza pneumonia.
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DOI：
10.1084/jem.177.2.475
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发表时间：
1993-02-01
期刊：
影响因子：
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通讯作者：
Doherty PC
中科院分区：
文献类型：
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作者：
Carding SR;Allan W;McMickle A;Doherty PC
采用原位杂交技术，对感染病毒的小鼠肺和纵隔淋巴结细胞进行了细胞因子基因表达模式的研究。在从两个解剖部位恢复的所有淋巴细胞亚群中都发现了大量转录活性的证据。H3N2病毒初次感染后细胞因子mRNA表达的动态变化符合这样一种观点，即最初的应答发生在区域淋巴组织中，效应性T细胞随后移动到肺部。这种时间间隔对于H3N2免疫的小鼠感染H1N1病毒所产生的更快的二次反应来说就不那么明显了。在T细胞受体α/β+亚群中，干扰素(干扰素)伽马和肿瘤坏死因子β的转录本在CD8+人群中最常见，而白介素4和白介素10的mRNA在CD4+T细胞中更常见。在炎性渗出液中恢复的T细胞中，IL-2、IL-4和干扰素-γ占主导地位。在特定的时间点，特别是在MLN早期和感染肺的晚期，mRNA+淋巴细胞的频率远远高于目前对病毒特异性前体和效应物流行的理解。如果这种反应是典型的，诱导对入侵病原体没有直接反应的T细胞的细胞因子基因表达可能是急性病毒感染的一个显著特征。
The patterns of cytokine mRNA expression in mice with primary or secondary influenza pneumonia have been assessed by in situ hybridization analysis of cells from both the mediastinal lymph node (MLN) and the virus-infected lung. Evidence of substantial transcriptional activity was found in all lymphocyte subsets recovered from both anatomical sites. The kinetics of cytokine mRNA expression after primary infection with an H3N2 virus were in accord with the idea that the initial response occurs in regional lymphoid tissue, with the effector T cells later moving to the lung. This temporal separation was much less apparent for the more rapid secondary response resulting from challenge of H3N2-primed mice with an H1N1 virus. Among the T cell receptor alpha/beta+ subsets, transcripts for interferon (IFN) gamma and tumor necrosis factor beta were most commonly found in the CD8+ population whereas mRNA for interleukin (IL) 4 and IL-10 was much more prevalent in CD4+ T cells. The gamma/delta T cells expressed mRNA for all cytokines tested, with IL-2, IL-4, and IFN-gamma predominating among those recovered from the inflammatory exudate. At particular time points, especially early in the MLN and late in the infected lung, the frequency of mRNA+ lymphocytes was much higher than would be expected from current understanding of the prevalence of virus-specific precursors and effectors. If this response is typical, induction of cytokine gene expression for T cells that are not responding directly to the invading pathogen may be a prominent feature of acute virus infections.