Wound healing effect of autologous fibrin glue and polyglycolic acid sheets in a rat back skin defect model
Wound healing effect of autologous fibrin glue and polyglycolic acid sheets in a rat back skin defect model
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DOI：
10.1016/j.transci.2021.103144
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发表时间：
2021-08-17
影响因子：
1.9
通讯作者：
Takahashi, Tetsu
中科院分区：
文献类型：
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作者：
Kouketsu, Atsumu;Shimizu, Yoshinaka;Takahashi, Tetsu
自体血浆生物胶可预防病毒感染和过敏反应。此外，这种生物材料含有生长因子，如TGF-β和VEGF，通过刺激成纤维细胞增殖和血管生成促进粘膜重建。因此，预计自体纤维蛋白胶比商业纤维蛋白胶更好地改善愈合。在这里，我们评估了自体纤维蛋白胶对伤口愈合关键早期阶段的影响。在大鼠中引入上皮缺损，并用聚乙醇酸（PGA）片覆盖，含或不含市售或自体纤维蛋白胶。通过组织学和免疫组织化学评估伤口愈合六周。我们的研究结果表明，用PGA片和自体纤维蛋白胶覆盖的伤口实现了有效的伤口愈合，没有并发症，如局部感染或愈合不完全。该组中再生上皮的恢复率上级优于PGA片和商业纤维蛋白胶覆盖的伤口。层粘连蛋白、细胞角蛋白和VEGF的免疫组化证实了上皮新生的精细和快速。总的来说，我们的研究结果表明，用自体纤维蛋白胶覆盖手术伤口可促进伤口愈合和上皮形成，提高安全性，并降低与传统技术相关的病毒感染和过敏反应的风险。
Fibrin glue from autologous plasma may prevent viral infection and allergic reaction. Moreover, this biomaterial contains growth factors such as TGF-beta and VEGF that promote reconstruction of the mucous membrane by stimulating fibroblast proliferation and angiogenesis. Thus, autologous fibrin glue is predicted to improve healing better than commercial fibrin glue. Here, we evaluated the effects of autologous fibrin glue on the crucial early phase of wound healing. Epithelial defects were introduced in rats and covered with polyglycolic acid (PGA) sheets with or without commercial or autologous fibrin glue. Wound healing was assessed for six weeks by histology and immunohistochemistry. Our results demonstrate that wounds covered with PGA sheets and autologous fibrin glue achieved efficient wound healing without complications such as local infection or incomplete healing. The rate of recovery of the regenerating epithelium in this group was superior to that in wounds covered with PGA sheets and commercial fibrin glue. Immunohistochemistry of laminin, cytokeratin, and VEGF confirmed fine and rapid epithelial neogenesis. Collectively, our results indicate that covering surgical wounds with autologous fibrin glue promotes wound healing and epithelialization, improves safety, and reduces the risks of viral infection and allergic reaction associated with conventional techniques.