Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors
Terahertz Imaging and Characterization Protocol for Freshly Excised Breast Cancer Tumors
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DOI：
10.3791/61007
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发表时间：
2020-04-01
影响因子：
1.2
通讯作者：
El-Shenawee, Magda
中科院分区：
文献类型：
--
作者：
Vohra, Nagma;Bowman, Tyler;El-Shenawee, Magda
这份手稿提出了一个协议，处理，表征，并使用脉冲太赫兹成像和光谱技术成像新鲜切除的人类乳腺肿瘤。该协议包括垂直入射的太赫兹透射模式和倾斜30度角的太赫兹反射模式。所收集的实验数据表示电场的时域脉冲。通过分析模型处理通过被切除组织上的固定点传输的太赫兹电场信号，以提取组织的折射率和吸收系数。利用步进电机扫描仪，太赫兹发射脉冲从肿瘤上的每个像素反射，提供不同组织区域的平面图像。图像可以在时域或频域中呈现。此外，利用在每个像素处提取的折射率和吸收系数的数据来提供肿瘤的断层太赫兹图像。该方案表明癌组织和健康组织之间存在明显差异。另一方面，不遵守协议可能会导致噪声或不准确的图像，因为气泡和液体残留在肿瘤表面上。该方案提供了一种评估乳腺肿瘤手术切缘的方法。
This manuscript presents a protocol to handle, characterize, and image freshly excised human breast tumors using pulsed terahertz imaging and spectroscopy techniques. The protocol involves terahertz transmission mode at normal incidence and terahertz reflection mode at an oblique angle of 30 degrees. The collected experimental data represent time domain pulses of the electric field. The terahertz electric field signal transmitted through a fixed point on the excised tissue is processed, through an analytical model, to extract the refractive index and absorption coefficient of the tissue. Utilizing a stepper motor scanner, the terahertz emitted pulse is reflected from each pixel on the tumor providing a planar image of different tissue regions. The image can be presented in time or frequency domain. Furthermore, the extracted data of the refractive index and absorption coefficient at each pixel are utilized to provide a tomographic terahertz image of the tumor. The protocol demonstrates clear differentiation between cancerous and healthy tissues. On the other hand, not adhering to the protocol can result in noisy or inaccurate images due to the presence of air bubbles and fluid remains on the tumor surface. The protocol provides a method for surgical margins assessment of breast tumors.