Expression of sclerostin in the developing zebrafish (Danio rerio) brain and skeleton.
Expression of sclerostin in the developing zebrafish (Danio rerio) brain and skeleton.
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DOI：
10.1016/j.gep.2012.04.003
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发表时间：
2012-08
期刊：
影响因子：
--
通讯作者：
Kumar R
中科院分区：
文献类型：
--
作者：
McNulty MS;Bedell VM;Greenwood TM;Craig TA;Ekker SC;Kumar R
硬化蛋白是一种高度保守的分泌型胱氨酸结蛋白，调节成骨细胞的功能。具有硬化蛋白基因（SOST）突变的人类表现出轴向和非轴向骨骼骨密度增加，伴随并发症。在成人骨中，硬化蛋白在骨细胞和成骨细胞中表达。斑马鱼类硬化蛋白与鲈鱼硬化蛋白密切相关，与鸡和哺乳动物的硬化蛋白也有一定的关系。关于sclerostin在早期发育的骨骼或骨骼外组织中的表达知之甚少。应用全胚胎原位杂交技术研究了斑马鱼（Danio rerio）胚胎发育过程中硬化蛋白（sost）基因的表达。sost RNA的最早表达出现在受精后12小时（hpf）。在15 hpf，sost RNA中检测到发育中的神经系统和枯否氏囊泡。在18，20和22 hpf，在菱形唇前体中观察到表达。到24 hpf时，在上下菱形唇和发育中的脊髓中观察到表达。在菱形唇和脊髓中的表达持续到28 hpf，然后在44 hpf时强度减弱。在28 hpf时，在发育中的咽软骨中注意到sost表达;咽软骨中的表达随时间增加。到48 hpf，在发育中的咽弓软骨中清楚地检测到sost RNA。Sost RNA在咽弓软骨和发育中的胸鳍、72、96和120 hpf中大量表达。我们的研究是第一个详细的分析sost基因表达的早期后生动物的发展。
Sclerostin is a highly conserved, secreted, cystine-knot protein which regulates osteoblast function. Humans with mutations in the sclerostin gene (SOST), manifest increased axial and appendicular skeletal bone density with attendant complications. In adult bone, sclerostin is expressed in osteocytes and osteoblasts. Danio rerio sclerostin-like protein is closely related to sea bass sclerostin, and is related to chicken and mammalian sclerostins. Little is known about the expression of sclerostin in early developing skeletal or extra-skeletal tissues. We assessed sclerostin (sost) gene expression in developing zebrafish (Danio rerio) embryos with whole mount is situ hybridization methods. The earliest expression of sost RNA was noted during 12 hours post-fertilization (hpf). At 15 hpf, sost RNA was detected in the developing nervous system and in Kupffer’s vesicle. At 18, 20 and 22 hpf, expression in rhombic lip precursors was seen. By 24 hpf, expression in the upper and lower rhombic lip and developing spinal cord was noted. Expression in the rhombic lip and spinal cord persisted through 28 hpf and then diminished in intensity through 44 hpf. At 28 hpf, sost expression was noted in developing pharyngeal cartilage; expression in pharyngeal cartilage increased with time. By 48 hpf, sost RNA was clearly detected in the developing pharyngeal arch cartilage. Sost RNA was abundantly expressed in the pharyngeal arch cartilage, and in developing pectoral fins, 72, 96 and 120 hpf. Our study is the first detailed analysis of sost gene expression in early metazoan development.
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影响因子：
39.2
作者：
BEIGHTON, P;DURR, L;HAMERSMA, H
通讯作者：
HAMERSMA, H
DOI：
10.1073/pnas.96.5.2439
发表时间：
1999-03-02
影响因子：
11.1
作者：
Golden, JA;Bracilovic, A;Grinspan, JB
通讯作者：
Grinspan, JB
影响因子：
4
作者：
Balemans, W;Patel, N;Van Hul, W
通讯作者：
Van Hul, W
影响因子：
4.8
作者：
Kusu, N;Laurikkala, J;Itoh, N
通讯作者：
Itoh, N
影响因子：
3.4
作者：
Itin, PH;Keserü, B;Hauser, V
通讯作者：
Hauser, V