Increased levels of the oxidative stress marker, nitrotyrosine in patients with provocation test-induced coronary vasospasm
Increased levels of the oxidative stress marker, nitrotyrosine in patients with provocation test-induced coronary vasospasm
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DOI：
10.1016/j.jjcc.2013.11.017
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发表时间：
2014-08-01
影响因子：
2.5
通讯作者：
Kajinami, Kouji
中科院分区：
文献类型：
--
作者：
Tanabe, Kan;Kawai, Yasuyuki;Kajinami, Kouji
背景：氧化应激引起的冠状动脉内皮功能障碍在冠状动脉痉挛的发病机制中起重要作用。然而，尚不清楚冠状血管痉挛后氧化应激的循环生物标志物是否发生改变。我们研究了由冠状动脉内乙酰胆碱激发试验引起的冠状血管痉挛导致短暂性心肌缺血后氧化应激生物标志物水平的时间变化。方法与结果：连续30例疑似血管痉挛型心绞痛（VSAP）患者入组研究。根据检测结果将患者分为vsap阳性组（n = 14）和vsap阴性组（n = 16）。测定小鼠血清中氧化应激标志物4-羟基壬烯醛（HNE）和硝基酪氨酸（NT）在激发试验前、15 min、3 h和12 h的水平。两组检测后血清HNE水平均无变化。与基线（8.1 +/- 3.2 mu g/ml）相比，vsap阳性组血清NT水平在试验后3 h和12 h显著升高（3 h时为11.3 +/- 3.3 mu g/ml, p = 0.015, 12 h时为12.1 +/- 5.7 mu g/ml, p = 0.03）。在vsap阴性组，血清NT水平在3个时间点均较基线显著下降。结论：乙酰胆碱诱发冠状动脉痉挛后血清NT明显升高，提示血清NT可能是一过性心肌缺血的生物标志物，参与了VSAP的发生发展。(C) 2014年由Elsevier Ltd代表日本心脏病学院出版。
Background: Endothelial dysfunction of the coronary arteries caused by oxidative stress plays an important role in the pathogenesis of coronary vasospasm. However, it is not clear whether circulating biomarkers for oxidative stress are altered after coronary vasospasm. We investigated temporal changes in the levels of oxidative stress biomarkers after coronary vasospasm induced by intracoronary acetylcholine provocation testing, resulting in transient myocardial ischemia.Methods and results: Thirty consecutive patients with suspected vasospastic angina pectoris (VSAP) were enrolled in the study. Patients were categorized into the VSAP-positive group (n = 14) and the VSAP-negative group (n = 16) on the basis of test results. Serum samples were examined for the levels of the oxidative stress markers 4-hydroxynonenal (HNE) and nitrotyrosine (NT) before, and 15 min, 3 h, and 12 h after the provocation test. The serum HNE levels did not change in either group after the test. The serum NT levels in the VSAP-positive group significantly increased at 3 h and 12 h after the test (11.3 +/- 3.3 mu g/ml at 3 h, p = 0.015, and 12.1 +/- 5.7 mu g/ml at 12 h, p = 0.03), as compared with baseline (8.1 +/- 3.2 mu g/ml). In the VSAP-negative group, the serum NT levels significantly decreased from baseline at each of the 3 time points.Conclusions: Serum NT significantly increased after coronary vasospasm induced by acetylcholine provocation, suggesting that serum NT could be a biomarker of transient myocardial ischemia and could contribute to the development of VSAP. (C) 2014 Published by Elsevier Ltd on behalf of Japanese College of Cardiology.