Endogenous apurinic/apyrimidinic sites in genomic DNA of mammalian tissues.
Endogenous apurinic/apyrimidinic sites in genomic DNA of mammalian tissues.
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发表时间：
1999-06
期刊：
影响因子：
11.2
通讯作者：
Jun Nakamura;J. Swenberg
中科院分区：
文献类型：
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作者：
Jun Nakamura;J. Swenberg
脱嘌呤/脱嘧啶（AP）位点是DNA中最常见的损伤之一。采用高灵敏度的狭缝印迹法，测定了大鼠和人肝正常组织中内源性AP位点的数量和状态。AP位点的数量（每个哺乳动物细胞50，000 - 200，000个）在大脑中最多，其次是结肠和心脏，然后是肝脏，肺和肾脏。大多数内源性AP位点在AP位点的5'端被切割。这些数据表明，去除脱氧核糖磷酸部分是在体内的碱基切除和AP位点修复的限速步骤。
Apurinic/apyrimidinic (AP) sites are one of the most frequent lesions in DNA. Using a highly sensitive slot blot assay, we determined the number and condition of endogenous AP sites in normal tissues of rats and human liver. The number of AP sites (50,000-200,000 per mammalian cell) was greatest in brain, followed by colon and heart, and then liver, lung, and kidney. The majority of endogenous AP sites were cleaved 5' to the AP site. These data suggest that removal of the deoxyribosyl phosphate moiety is the rate-limiting step in base excision and AP site repair in vivo.