Cooperative inputs of Bmp and Fgf signaling induce tail regeneration in urodele amphibians
Cooperative inputs of Bmp and Fgf signaling induce tail regeneration in urodele amphibians
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DOI：
10.1016/j.ydbio.2015.12.012
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发表时间：
2016-02-01
影响因子：
2.7
通讯作者：
Satoh, Akira
中科院分区：
文献类型：
--
作者：
Makanae, Aki;Mitogawa, Kazumasa;Satoh, Akira
有尾目两栖动物具有显著的器官再生能力。它们一生中不仅可以再生四肢，还可以再生尾巴。已经证明，某些器官的再生是由神经组织的存在所控制的。例如，没有神经就不能诱导肢体再生。因此，确定神经因子已成为两栖动物器官再生研究的主要焦点。最近，在肢体再生中的神经替代分子BMP和Fgfs被鉴定。BMP和Fgfs的协同输入可以在没有神经的情况下诱导肢体再生。在本研究中，我们研究了相似或相同的再生机制是否控制另一种神经组织支配的器官再生，即，尾再生，在Ambystoma墨西哥。尾部的神经组织，也就是脊髓，可以将伤口愈合反应转化为器官再生反应，类似于肢体再生中的神经。此外，所鉴定的再生诱导剂Fgf 2 + Fgf 8 + Bmp 7表现出类似的诱导效果。然而，进一步的分析表明，由Fgf2+Fgf8+Bmp7诱导的芽基细胞可以参与多种组织的再生，但不能组织图案化的尾部。Fgf 2 + Fgf 8 + Bmp 7在另一种有尾目动物（Pleurodeles waltl）中证实了再生诱导能力。这些结果表明，有尾目两栖动物的器官再生能力是由一个共同的机制控制。(C)2015年，作者。爱思唯尔公司出版
Urodele amphibians have remarkable organ regeneration ability. They can regenerate not only limbs but also a tail throughout their life. It has been demonstrated that the regeneration of some organs are governed by the presence of neural tissues. For instance, limb regeneration cannot be induced without nerves. Thus, identifying the nerve factors has been the primary focus in amphibian organ regeneration research. Recently, substitute molecules for nerves in limb regeneration, Bmp and Fgfs, were identified. Cooperative inputs of Bmp and Fgfs can induce limb regeneration in the absence of nerves. In the present study, we investigated whether similar or same regeneration mechanisms control another neural tissue governed organ regeneration, i.e., tail regeneration, in Ambystoma mexicanum. Neural tissues in a tail, which is the spinal cord, could transform wound healing responses into organ regeneration responses, similar to nerves in limb regeneration. Furthermore, the identified regeneration inducer Fgf2+Fgf8+Bmp7 showed similar inductive effects. However, further analysis revealed that the blastema cells induced by Fgf2+Fgf8+Bmp7 could participate in the regeneration of several tissues, but could not organize a patterned tail. Regeneration inductive ability of Fgf2+Fgf8+Bmp7 was confirmed in another urodele, Pleurodeles waltl. These results suggest that the organ regeneration ability in urodele amphibians is controlled by a common mechanism. (C) 2015 The Authors. Published by Elsevier Inc.