AN INVESTIGATION ON THE ROLE OF PLASMA AND SERUM OPSONINS ON THE INTERNALIZATION OF BIODEGRADABLE POLY(D,L-LACTIC ACID) NANOPARTICLES BY HUMAN MONOCYTES
AN INVESTIGATION ON THE ROLE OF PLASMA AND SERUM OPSONINS ON THE INTERNALIZATION OF BIODEGRADABLE POLY(D,L-LACTIC ACID) NANOPARTICLES BY HUMAN MONOCYTES
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DOI：
10.1016/0024-3205(95)00321-v
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发表时间：
1995-07-07
期刊：
影响因子：
6.1
通讯作者：
GURNY, R
中科院分区：
文献类型：
--
作者：
LEROUX, JC;DEJAEGHERE, F;GURNY, R
我们在这里证明，聚乙二醇（PEG）6，000保护可生物降解的聚（D，L-乳酸）纳米颗粒（PLA NP）免受血浆中单核细胞的广泛摄取。这些结果与先前用PEG 20，000获得的结果一致，PEG 20，000减少了人单核细胞在磷酸盐缓冲盐水和血浆中对PLA NP的摄取，并延长了NP在体内的循环时间。PEG 6，000和20，000的包被效率在血清中显著降低。对于PEG 20，000包被的NP，在热灭活血清和IgG耗尽血清中，普通NP和包被NP的摄取之间的差异清楚地再次出现。我们认为，典型的血浆蛋白，热不稳定血清蛋白（如补体成分）和IgG参与了平原和涂层PLA NP的调理作用。以前发现吸附到这些NP上的其他蛋白质，即白蛋白和载脂蛋白E，似乎没有直接影响摄取过程。
We demonstrate here that polyethylene glycol (PEG) 6,000 protects biodegradable poly(D,L-lactic acid) nanoparticles (PLA NP) from extensive uptake by monocytes in plasma. These results are in agreement with those previously obtained with PEG 20,000 which reduced the uptake of PLA NP by human monocytes in phosphate buffered saline and plasma, and prolonged the NP circulation time in vivo. The coating efficiency of PEG 6,000 and 20,000 was substantially decreased in serum. The difference between the uptake of plain and coated NP clearly reappeared for PEG 20,000-coated NP in heat inactivated serum and in IgG-depleted serum. We suggest that typical plasma proteins, heat labile serum proteins (e.g. complement components) and IgG are involved in the opsonization of plain and coated PLA NP. Other proteins previously found to adsorb onto these NP, namely albumin and apolipoprotein E, did not appear to directly influence the uptake process.