Influence of Dimethyl Sulphoxide on Glycine Transport in Sarcoma-180 Tumour Cells
Influence of Dimethyl Sulphoxide on Glycine Transport in Sarcoma-180 Tumour Cells
复制标题
二甲亚砜对 Sarcoma-180 肿瘤细胞中甘氨酸转运的影响
作者：
R. Hagemann;T. Evans
二甲基亚砜 (DMSO) 抑制许多生物合成过程；成纤维细胞1和细菌2的生长、肉瘤180肿瘤细胞3中的DNA合成、甲状腺转运和碘的组织（我们未发表的观察结果）以及蛋白质合成4,5在体外均被足够高浓度的该化合物抑制。在相似浓度的 DMSO 中，许多酶的活性也会降低。一个有趣的事实是，DMSO 的这些抑制作用是可逆的，可以通过充分降低 DMSO 浓度来克服它们，例如通过用生理盐水“清洗”组织、细胞或酶。 DMSO 抑制的机制尚不清楚，可能涉及非特异性酶抑制，尽管某些酶的活性在 DMSO6 中增加。
DIMETHYL sulphoxide (DMSO) inhibits a number of biosynthetic processes; growth of fibroblasts1 and bacteria2, DNA synthesis in sarcoma-180 tumour cells3, thyroidal transport and organification of iodine (our unpublished observations), and protein systhesis4,5 are all inhibited by sufficiently high concentrations of this compound in vitro. The activity of a number of enzymes6 is also reduced in similar concentrations of DMSO. An interesting fact is that these inhibitory effects of DMSO are reversible—they can be overcome by a sufficient reduction in the DMSO concentration, for example, by “washing” the tissue, cells or enzyme in physiological saline. The mechanism of DMSO inhibition, which is unknown, may involve non-specific enzyme inhibition, although the activity of some enzymes is increased in DMSO6.