Detection of albumin using skin blotting as a measure of skin barrier function
Detection of albumin using skin blotting as a measure of skin barrier function
复制标题
使用皮肤印迹检测白蛋白作为皮肤屏障功能的测量
DOI：
10.24462/jnse.4.2_116
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
H. Sanada
中科院分区：
文献类型：
--
作者：
Nao Tamai;T. Minematsu;Sanai Tsunokuni;Kumiko Aso;Shiho Higashimura;G. Nakagami;H. Sanada
经皮水分丢失（TEWL）的测量是评价皮肤屏障功能的金标准。然而，在临床环境中很难获得稳定的值。在这项研究中，我们评估了使用皮肤印迹法检测白蛋白作为一种新的测量方法来确定皮肤屏障功能。通过动物模型和人体皮肤印迹法分析TEWL值之间的相关性并检测白蛋白染色强度。白蛋白强度与小鼠背部皮肤样品中的TEWL值显著相关（r = 0.56，p=0.02）。此外，白蛋白强度还与人皮肤中的TEWL值相关（r=0.73，p < 0.01）。因此，使用皮肤印迹法的白蛋白强度是一种非侵入性和稳定的方法，用于评估皮肤屏障功能，作为TEWL的替代方法。
Measurement of transepidermal water loss(TEWL)is the gold standard for the evaluation of skin barrier function. However, it is difficult to obtain stable values in the clinical setting. In this study, we evaluated the use of albumin detection by skin blotting as a new measurement method for determination of skin barrier function. We analyzed the correlation between TEWL values and detected the intensity of albumin staining by skin blotting in animal models and humans. The albumin intensities were significantly correlated with TEWL values in samples of back skin from mice(r = 0.56, p=0.02). In addition, the albumin intensities were also correlated with TEWL values in human skin(r=0.73, p < 0.01). Thus, albumin intensity using skin blotting was a noninvasive and stable method for evaluating skin barrier function as an alternative to TEWL.
影响因子：
3.1
作者：
Koyano, Yuiko;Nakagami, Gojiro;Sanada, Hiromi
通讯作者：
Sanada, Hiromi