DISTRIBUTION OF SUBSTANCE-P AND CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVE NERVE-FIBERS IN THE RAT TEMPOROMANDIBULAR-JOINT
DISTRIBUTION OF SUBSTANCE-P AND CALCITONIN GENE-RELATED PEPTIDE-LIKE IMMUNOREACTIVE NERVE-FIBERS IN THE RAT TEMPOROMANDIBULAR-JOINT
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DOI：
10.1177/00220345930720030701
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发表时间：
1993-03-01
影响因子：
7.6
通讯作者：
TANAKA, T
中科院分区：
文献类型：
--
作者：
KIDO, MA;KIYOSHIMA, T;TANAKA, T
应用免疫组织化学方法，观察了大鼠颞下颌关节（TMJ）内P物质样免疫反应（SP LI）和降钙素基因相关肽样免疫反应（CGRP LI）神经纤维的密度和分布，发现SP LI和CGRP LI神经纤维主要分布于关节囊、关节盘周围、滑膜、关节囊、关节盘周围、关节囊、关节囊、关节盘周围、关节囊、关节盘周围、关节囊、关节囊、关节盘周围、关节盘周围、关节盘和骨膜CGRP阳性神经的分布与SP阳性神经相似。关节囊和椎间盘的前部神经支配最密集，其次是后部、外侧和内侧部分。CGRP阳性神经较SP阳性神经数量多、密度高。在滑膜中，许多SP和CGRP样神经终止于滑膜下层，但也有分支延伸到靠近关节腔的浅表滑膜衬里层。免疫标记的神经主要位于椎间盘附件和椎间盘的外周部分，偶尔神经位于致密的胶原椎间盘带中，作为一个实际的椎间盘。然而，在中央盘带中未检测到纤维。因此，大部分的光盘是不受任何nerve.The本研究提供了一个形态学基础的神经肽的可能作用，内吞的滑膜细胞，调节血液流动的滑膜，伤害性的TMJ的机制，和调制的炎症反应在TMJ。
The density and distribution of substance P-like immunoreactive (SP-LI) and calcitonin gene-related peptide-like immunoreactive (CGRP-LI) nerve fibers in rat temporomandibular joint (TMJ) were investigated in whole-mount preparations and frozen sections by immunohistochemistry with the avidin-biotin-peroxidase complex method.Both types of immunoreactive nerves were observed primarily in the joint capsule, the peripheral articular disc, the synovial membrane, and the periosteum. The distribution of CGRP-LI nerves was similar to that of SP-LI nerves. The anterior portion of the joint capsule and disc was most densely innervated, followed by the posterior, lateral, and medial portions. In addition, CGRP-LI nerves were more numerous and more dense in immunointensity than SP-LI nerves. In the synovial membrane, many SP- and CGRP-LI nerves terminated in the subsynovial layer, but some branches extended into the superficial synovial lining layer close to the joint cavity. Immunolabeled nerves were prominently located in the disc attachment and peripheral portion of the disc, and occasional nerves were located in the dense collagenous disc band as an actual disc. However, no fibers were detected in the central disc band. Thus, most of the disc was not innervated by any nerves.The present study provides a morphological basis for the possible roles of neuropeptides in endocytosis by synoviocytes, regulation of blood flow in the synovial membrane, nociception mechanisms of the TMJ, and modulation of the inflammatory response in the TMJ.