Orally administered lactoferrin restores humoral immune response in immunocompromised mice
Orally administered lactoferrin restores humoral immune response in immunocompromised mice
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DOI：
10.1016/s0165-2478(03)00102-0
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发表时间：
2003-10-09
影响因子：
4.4
通讯作者：
Kruzel, ML
中科院分区：
文献类型：
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作者：
Artyrn, J;Zimecki, M;Kruzel, ML
环磷酰胺（CP）是一种抗肿瘤药物，常用于人类癌症和自身免疫性疾病的化疗。在我们以前的研究中，我们已经证明，乳铁蛋白（LF），口服CP免疫抑制小鼠，可以重建T细胞介导的免疫反应的T细胞群的更新。本研究的目的是评估LF对环磷酰胺处理的小鼠的不道德反应的影响。我们证明，一个单一的，亚致死剂量的环磷酰胺（400毫克/公斤体重）深刻抑制CBA小鼠的体液免疫反应，绵羊红细胞（SRBC），作为测量的抗体形成细胞（AFC）的数量在脾脏后5周CP治疗。在饮用水中给予0.5%牛LF 5周，部分重建了AFC值（对照值的30-40%，但比CP处理对照高7-10倍）。流式细胞术测定脾脏T和B细胞水平显示，LF治疗后免疫功能低下小鼠的CD 3（+）和CD 4（+）以及IG（+）脾细胞含量升高。此外，腹腔巨噬细胞的数量在LF处理后部分恢复。对刀豆球蛋白A（ConA）和美洲商陆有丝分裂原（PWM）的增殖反应的评价表明，LF显着增强了CP处理小鼠脾细胞的反应性降低。总之，我们的结论是，长期口服LF治疗免疫功能低下的小鼠导致体液反应的部分重建，与T和B细胞和巨噬细胞含量的升高和脾细胞对有丝分裂原的增殖反应有关。(C)2003 Elsevier Science B. V.保留所有权利。
Cyclophosphamide (CP) is an anti-tumor drug commonly used in the chemotherapy of human cancer and autoimmune diseases. In our previous studies, we have demonstrated that lactoferrin (LF), given orally to CP-immunosuppressed mice, could reconstitute a T cell mediated immune response by the renewal of the T cell population. The aim of this present study was to evaluate the effects of LF on Immoral responses in mice treated with cyclophosphamide. We demonstrate that a single, sublethal dose of cyclophosphamide (400 mg/kg body weight) profoundly inhibited the humoral immune response of CBA mice to sheep red blood cells (SRBC), as measured by the number of antibody forming cells (AFC) in the spleen after 5 weeks following CP treatment. Administration of 0.5% bovine LF in drinking water for 5 weeks partially reconstituted the AFC number (30-40% of the control values, but 7-10 x more than in CP-treated controls). Determination of T and B cell levels in the spleens by flow cytometry revealed that the content of CD3(+) and CD4(+) as well as Ig(+) splenocytes was elevated in the immunocompromised mice treated with LF. In addition, the number of peritoneal macrophages was partially restored following LF treatment. Evaluation of the proliferative response to concanavalin A (ConA) and pokeweed mitogen (PWM) demonstrated that the diminished reactivity of splenocytes from CP-treated mice was significantly enhanced by LF. In summary, we conclude that the prolonged, oral treatment of immunocompromised mice with LF led to partial reconstitution of the humoral response, associated with elevation of T and B cell and macrophage content and the proliferative response of splenocytes to mitogens. (C) 2003 Elsevier Science B.V. All rights reserved.