Regulation of the TMEPAI promoter by TCF7L2: the C-terminal tail of TCF7L2 is essential to activate the TMEPAI gene
Regulation of the TMEPAI promoter by TCF7L2: the C-terminal tail of TCF7L2 is essential to activate the TMEPAI gene
复制标题
DOI：
10.1093/jb/mvv117
复制
发表时间：
2016-01-01
影响因子：
2.7
通讯作者：
Itoh, Susumu
中科院分区：
文献类型：
--
作者：
Nakano, Naoko;Kato, Mitsuyasu;Itoh, Susumu
我们之前发现TCF7L2可以有效激活TMEPAI基因，而LEF1几乎不能增强其转录。当我们理解 TCF7L2 和 LEF1 在 TMEPAI 基因激活方面的功能差异时，需要 TCF7L2 的 C 末端尾部来揭示其转录活性及其与 Smad3 的相互作用。一致地，TCF7/TCF7L2 和 LEF1/TCF7L2 嵌合蛋白在转录和 Smad3 结合方面表现出与 TCF7L2 相似的活性，与 LEF1 和 TCF7 相比。我们的数据详细阐述了 TCF/LEF 家族成员在 TMEPAI 基因转录调控方面的不同活性。
We previously found that TCF7L2 could activate the TMEPAI gene efficiently, whereas LEF1 could not nearly augment its transcription. When we comprehended the functional difference(s) between TCF7L2 and LEF1 with respect to the activation of the TMEPAI gene, the C-terminal tail of TCF7L2 was needed to reveal its transcriptional activity as well as its interaction with Smad3. Consistently, both TCF7/TCF7L2 and LEF1/TCF7L2 chimeric proteins exhibited an activity similar to TCF7L2 in transcription and Smad3 binding in contrast with LEF1 and TCF7. Our data elaborated on the diverse activity among TCF/LEF family members with respect to the transcriptional regulation of the TMEPAI gene.