THE INITIATOR AS A TRANSCRIPTION CONTROL ELEMENT
THE INITIATOR AS A TRANSCRIPTION CONTROL ELEMENT
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DOI：
10.1016/0092-8674(89)90176-1
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发表时间：
1989-04-07
期刊：
影响因子：
64.5
通讯作者：
BALTIMORE, D
中科院分区：
文献类型：
--
作者：
SMALE, ST;BALTIMORE, D
淋巴细胞特异性末端脱氧核苷酸转移酶基因的转录始于一个核苷酸，但不存在TATA盒。我们已经确定了一个17 bp的元件，这是足够的准确的基础转录该基因在体外和体内。该基序，起始子（Inr），在其自身内包含转录起始位点。在许多含TATA的基因中发现了与Inr的同源性，并且特异性诱变影响起始的效率和准确性。此外，在TATA盒或SV40 21 bp重复序列的存在下，转录水平显著增加，特异性地在Inr起始。因此，Inr构成了已鉴定的最简单的功能性启动子，并为缺乏TATA元件的启动子如何指导转录起始提供了一种解释。
Transcription of the lymphocyte-specific terminal deoxynucleotidyltransferase gene begins at a single nucleotide, but no TATA box is present. We have identified a 17 bp element that is sufficient for accurate basal transcription of this gene both in vitro and in vivo. This motif, the initiator (Inr), contains within itself the transcription start site. Homology to the Inr is found in many TATA-containing genes, and specific mutagenesis influences both the efficiency and accuracy of initiation. Moreover, in the presence of either a TATA box or the SV40 21 bp repeats, a greatly increased level of transcription initiates specifically at the Inr. Thus, the Inr constitutes the simplest functional promoter that has been identified and provides one explanation for how promoters that lack TATA elements direct transcription initiation.