Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research.
Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research.
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DOI：
10.1038/s41598-023-35723-2
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发表时间：
2023-05-31
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
支气管肺泡灌洗(BAL)正成为传染病免疫学研究的常用方法。对影响手术主要结果的临床因素知之甚少。在根据指南接受BAL的研究参与者中，分析了BAL体积产量、细胞产量、浓度、活力、颗粒颜色和差异计数与重要参与者特征的关系，这些特征包括活动性结核病、结核病暴露、艾滋病毒感染和最近的SARS-CoV-2感染。在337名参与者中，活动性结核病患者和当前吸烟者的BAL体积和BAL细胞计数相关。右肺中叶的体积最大。年龄较大的参与者的BAL细胞和体积产量较低，他们的中性粒细胞也较多。现在的吸烟者产生的所有细胞类型的体积较小，数量较多，并且通常有一个黑色的颗粒。活动性结核病与更高的细胞产量有关，但在治疗结束时这一比例下降。HIV感染与更多的血球相关，以及最近感染SARS-CoV-2的淋巴细胞比例更高。这些结果使研究人员能够优化他们的参与者，并结束涉及肺免疫细胞的项目的分析选择。
Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, but this declined at the end of treatment. HIV infection was associated with more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells.
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支气管肺泡灌洗（BAL）用于研究；获得足够的样品产量。
DOI：
10.3791/4345
发表时间：
2014-03-24
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Collins AM;Rylance J;Wootton DG;Wright AD;Wright AK;Fullerton DG;Gordon SB
通讯作者：
Gordon SB
影响因子：
4.6
作者：
Koda K;Hozumi H;Yasui H;Suzuki Y;Karayama M;Furuhashi K;Enomoto N;Fujisawa T;Inui N;Nakamura Y;Suda T
通讯作者：
Suda T
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.4
作者：
Mair, Patrick;Wilcox, Rand
通讯作者：
Wilcox, Rand
DOI：
10.1164/rccm.201202-0320st
发表时间：
2012-05-01
影响因子：
24.7
作者：
Meyer, Keith C.;Raghu, Ganesh;Wood, Brent
通讯作者：
Wood, Brent