Bacterium-Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression
Bacterium-Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression
复制标题
细菌表达的 dsRNA 下调家蚕微孢子虫基因表达
DOI：
10.1111/jeu.12346
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发表时间：
2017-03-01
影响因子：
2.2
通讯作者：
Xu, Jinshan
中科院分区：
文献类型：
--
作者：
Pan, Qiuling;Wang, Ling;Xu, Jinshan
The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double-strand RNAs targeting the gene encoding ADP/ATP protein in N.bombycis. The results showed that dsRNAs deferentially suppressed the gene expression during N.bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E.coli to control the pebrine disease of the domestic silkworm.
