Effect of pyridoxine and pyridoxal on the in vitro growth of human malignant melanoma.
Effect of pyridoxine and pyridoxal on the in vitro growth of human malignant melanoma.
复制标题
吡哆醇和吡哆醛对人恶性黑色素瘤体外生长的影响。
作者：
T. Shultz;Santamaria Ag;D. Gridley;Dwight R. Stickney;James M. Slater
研究了补充维生素 B-6 对人类恶性黑色素瘤细胞系 (M21-HPB) 生长的体外影响。将不同浓度的吡哆醇 (PN) 或吡哆醛 (PL) 添加到细胞培养物中并孵育 12 天。 PL 的药理学水平（0.25-0.5 mM）导致细胞增殖显着减少。生理水平（0.005 mM）不会阻碍生长。与 PN 一起孵育的细胞显示出生长刺激。在暴露于药理学 PL (0.05-0.5 mM) 浓度的细胞中，细胞内 PL 和 5'-磷酸吡哆醛 (PLP) 水平升高，但 PN 没有升高。这些数据表明 PL 或 PLP 可能参与调节黑色素瘤细胞的生长，并且维生素 B-6 可能具有作为抗肿瘤剂的潜在用途。
The in vitro effect of vitamin B-6 supplementation on the growth of a human malignant melanoma cell line (M21-HPB) was investigated. Varying concentrations of pyridoxine (PN) or pyridoxal (PL) were added to cell cultures and incubated for 12 days. Pharmacologic levels of PL (0.25-0.5 mM) resulted in significant reductions in cell proliferation. Physiologic levels (0.005 mM) did not retard growth. Cells incubated with PN showed growth stimulation. Intracellular levels of PL and pyridoxal 5'-phosphate (PLP) were increased in cells exposed to pharmacologic PL (0.05-0.5 mM) concentrations, but not PN. These data suggest that PL or PLP may be involved in regulating the growth of melanoma cells and that vitamin B-6 may have potential use as an antineoplastic agent.