Gut Microbial Composition and Function Are Altered in Patients with Early Rheumatoid Arthritis
Gut Microbial Composition and Function Are Altered in Patients with Early Rheumatoid Arthritis
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DOI：
10.3390/jcm8050693
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发表时间：
2019-05-01
影响因子：
3.9
通讯作者：
Ji, Geun Eog
中科院分区：
文献类型：
--
作者：
Jeong, Yunju;Kim, Ji-Won;Ji, Geun Eog
类风湿性关节炎（RA）是一种以关节滑膜炎症和关节外表现为特征的自身免疫性疾病。最近的研究表明微生物影响类风湿关节炎的发病机制。然而，很少有研究检查早期RA患者，特别是女性患者的微生物分布。在本研究中，我们研究了早期RA女性患者的肠道微生物群特征和微生物功能，包括临床前和临床表现明显的RA病例。利用QIIME （quantitative insights into microbial ecology）和PICRUSt （reconstruction of unserved states）分析各组微生物多样性、组成和功能的变化。结果显示，与健康受试者相比，早期RA患者的生态失调是由于多样性减少。健康受试者与早期RA患者在门至属各分类群的微生物分布有显著差异。早期RA患者中富集拟杆菌门，而健康受试者中富集放线菌门，包括Collinsella属。基于同源群簇的功能分析显示，来自革兰氏阳性菌的与甲基萘醌生物合成相关的基因在健康受试者中富集，而铁转运相关基因在早期RA患者中富集。与脂多糖生物合成相关的基因，革兰氏阴性细菌内毒素，在临床表现明显的RA患者中富集。健康受试者和早期RA患者肠道微生物群在微生物多样性、分类群和相关功能上的明显差异，突出了肠道微生物群在RA早期阶段的参与。
Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation of the joints and extra-articular manifestations. Recent studies have shown that microorganisms affect RA pathogenesis. However, few studies have examined the microbial distribution of early RA patients, particularly female patients. In the present study, we investigated the gut microbiome profile and microbial functions in early RA female patients, including preclinical and clinically apparent RA cases. Changes in microbiological diversity, composition, and function in each group were analyzed using quantitative insights into microbial ecology (QIIME) and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt). The results revealed the dysbiosis due to decreased diversity in the early RA patients compared with healthy subjects. There were significant differences in the microbial distribution of various taxa from phylum to genus levels between healthy subjects and early RA patients. Phylum Bacteroidetes was enriched in early RA patients, while Actinobacteria, including the genus Collinsella, was enriched in healthy subjects. Functional analysis based on clusters of orthologous groups revealed that the genes related to the biosynthesis of menaquinone, known to be derived from gram-positive bacteria, were enriched in healthy subjects, while iron transport-related genes were enriched in early RA patients. Genes related to the biosynthesis of lipopolysaccharide, the gram-negative bacterial endotoxin, were enriched in clinically apparent RA patients. The obvious differences in microbial diversity, taxa, and associated functions of the gut microbiota between healthy subjects and early RA patients highlight the involvement of the gut microbiome in the early stages of RA.