Interactions between entomopathogenic fungi and Tamarixia radiata (Hymenoptera: Eulophidae) in Diaphorina citri (Hemiptera: Liviidae) populations under laboratory conditions
Interactions between entomopathogenic fungi and Tamarixia radiata (Hymenoptera: Eulophidae) in Diaphorina citri (Hemiptera: Liviidae) populations under laboratory conditions
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DOI：
10.1007/s10340-017-0870-z
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发表时间：
2018-01-01
影响因子：
4.8
通讯作者：
Robles-Bermudez, Agustin
中科院分区：
文献类型：
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作者：
Ibarra-Cortes, Karla H.;Gonzalez-Hernandez, Hctor;Robles-Bermudez, Agustin
利用拟寄生放射叶蝉或昆虫病原真菌生物防治柑橘斑潜蝇已有尝试，但很少有文献报道两者联合使用时的潜在相互作用。本文研究了寄主时间对辐射金龟子与柑橘白僵菌和金龟子绿僵菌相互作用结果的影响。实验一将感染真菌的若虫分别在0、24和72h后暴露于寄生蜂中，然后对不同发育阶段(卵、幼虫或蛹)的寄生蜂进行真菌接种。感染真菌的若虫比例最高的是在寄生蜂暴露前72h接种。寄生蜂暴露前72 h感染真菌的若虫寄生率最低。无论真菌感染有多严重，寄生蜂用于寄主取食的若虫数量都是相似的。对寄生在不同发育阶段的若虫进行真菌接种时，寄生有寄生虫卵的若虫感染比例最高。从对照和真菌感染处理中羽化的成虫寿命基本相同，但从接种寄生蜂幼虫的若虫中羽化的成虫寿命最低，对金龟子夜蛾的影响最大。讨论了从我们的结果中得出的生态重要性和实用建议。
Biological control of Diaphorina citri (Kuwayama) using the parasitoid Tamarixia radiata (Waterston) or entomopathogenic fungi has been attempted, but there have been few published studies on their potential interactions when used together. We studied the effect of prior residency time on the outcomes of interactions between T. radiata and the fungal pathogens Beauveria bassiana and Metarhizium anisopliae in D. citri nymphs. In the first experiment, nymphs infected with fungi were exposed to the parasitoid after 0, 24 and 72 h. Next, nymphs with parasitoids at different developmental stages (eggs, larvae or pupae) were exposed to fungal inoculation. The greatest proportion of fungus-infected nymphs occurred when they were inoculated 72 h prior to parasitoid exposure. The lowest parasitism rate occurred in nymphs that had been infected by fungi 72 h prior to parasitoid exposure. The number of nymphs used for host-feeding by the parasitoid was similar, regardless of how advanced the fungal infection was. When fungal inoculations were made to parasitized nymphs with different developmental stages of the parasitoid, the greatest proportion becoming infected occurred in nymphs with parasitoid eggs. The overall longevity of adult parasitoids emerging from control and fungal-infected treatments was similar; however, the longevity of adult parasitoids emerging from nymphs which had been inoculated with parasitoid larvae were lowest, with the greatest effect observed for the M. anisopliae isolate. The ecological importance and practical recommendations derived from our results are discussed.