Linking pulmonary oxygen uptake, muscle oxygen utilization and cellular metabolism during exercise
Linking pulmonary oxygen uptake, muscle oxygen utilization and cellular metabolism during exercise
复制标题
DOI：
10.1007/s10439-007-9271-4
复制
发表时间：
2007-06-01
影响因子：
3.8
通讯作者：
Cabrera, Marco E.
中科院分区：
文献类型：
--
作者：
Lai, Nicola;Camesasca, Marco;Cabrera, Marco E.
体育锻炼对氧运输和利用系统的组成部分施加的能量需求需要细胞和外部呼吸之间的密切联系，以维持ATP稳态。有创和无创的实验方法已被用来阐明运动过程中氧供应和消耗之间的平衡调节机制。这种方法表明，控制内部与外部呼吸耦合的各种子系统的机制是高度冗余和分层的多尺度系统的一部分。在这项工作中，我们提出了一个“系统生物学”的框架，它集成了实验和理论方法，能够同时提供可靠的信息，在氧气从空气到线粒体的途径中的各个步骤发生的氧气运输和利用过程，特别是在运动开始时。这个多学科框架提供了对运动过程中肌肉氧合测量得出的细胞耗氧量与间接测热法得出的肺摄氧量之间关系的见解。利用经过验证的模型，模拟了肌肉氧动力学响应，并在各种条件下与细胞代谢定量相关。
The energy demand imposed by physical exercise on the components of the oxygen transport and utilization system requires a close link between cellular and external respiration in order to maintain ATP homeostasis. Invasive and non-invasive experimental approaches have been used to elucidate mechanisms regulating the balance between oxygen supply and consumption during exercise. Such approaches suggest that the mechanism controlling the various subsystems coupling internal to external respiration are part of a highly redundant and hierarchical multi-scale system. In this work, we present a '' systems biology '' framework that integrates experimental and theoretical approaches able to provide simultaneously reliable information on the oxygen transport and utilization processes occurring at the various steps in the pathway of oxygen from air to mitochondria, particularly at the onset of exercise. This multi-disciplinary framework provides insights into the relationship between cellular oxygen consumption derived from measurements of muscle oxygenation during exercise and pulmonary oxygen uptake by indirect calorimetry. With a validated model, muscle oxygen dynamic responses is simulated and quantitatively related to cellular metabolism under a variety of conditions.