Swimming force and behavior of optically trapped micro-organisms
Swimming force and behavior of optically trapped micro-organisms
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DOI：
10.1364/optica.394232
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发表时间：
2020-08-20
期刊：
影响因子：
10.4
通讯作者：
Rubinsztein-Dunlop, Halina
中科院分区：
文献类型：
--
作者：
Armstrong, Declan J.;Nieminen, Timo A.;Rubinsztein-Dunlop, Halina
我们演示了如何结合三维力检测系统和高速摄像机的光镊被用来研究被困微生物的游泳力和行为。利用位置敏感探测器，我们测量了被捕获粒子的运动力，而不考虑其取向。这具有不需要复杂的光束成形或微流体控制来使捕获的颗粒在特定取向上对准的优点，从而导致在任何时间对推进力的明确测量。将直接测得的力与来自高速摄像机的位置数据相关联，使我们能够确定粒子行为的变化。我们通过测量大肠杆菌（E. coli）的游泳力并观察其游泳和翻滚模式之间的区别来展示我们的技术。coli）菌株MC 4100。我们的方法显示出应用前景，在未来的研究中可捕获的，但在其他任意形状的生物游泳者和其他活性物质。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
We demonstrate how optical tweezers combined with a three-dimensional force detection system and high-speed camera are used to study the swimming force and behavior of trapped micro-organisms. By utilizing position sensitive detec-tion, we measure the motility force of trapped particles, regardless of orientation. This has the advantage of not requiring complex beam shaping or microfluidic controls for aligning trapped particles in a particular orientation, leading to unambiguous measurements of the propulsive force at any time. Correlating the direct force measurements with posi-tion data from a high-speed camera enables us to determine changes in the particle's behavior. We demonstrate our technique by measuring the swimming force and observing distinctions between swimming and tumbling modes of the Escherichia coli (E. coli) strain MC4100. Our method shows promise for application in future studies of trappable but otherwise arbitrary-shaped biological swimmers and other active matter. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement