Effect of viral infection on host protein synthesis and mRNA association with the cytoplasmic cytoskeletal structure
Effect of viral infection on host protein synthesis and mRNA association with the cytoplasmic cytoskeletal structure
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病毒感染对宿主蛋白合成和 mRNA 与细胞质细胞骨架结构关联的影响
DOI：
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发表时间：
1985
影响因子：
7.8
通讯作者：
N. Sonenberg
中科院分区：
文献类型：
--
作者：
A. Bonneau;A. Darveau;N. Sonenberg
我们研究了几种真核病毒和细胞 mRNA 与源自正常和病毒感染细胞的细胞骨架部分的关联。我们发现，所有 mRNA 在蛋白质合成过程中似乎都与细胞骨架结构相关，无论其 5' 和 3' 末端结构如何：例如，缺乏 5' 帽结构的脊髓灰质炎病毒或呼肠孤病毒和组蛋白 mRNA 缺乏与细胞骨架框架相关的 3' 聚 A 尾，其程度与加帽的聚腺苷酸化肌动蛋白 mRNA 相同。当细胞被脊髓灰质炎病毒感染时，细胞（肌动蛋白）和病毒（水泡性口炎病毒和呼肠孤病毒）mRNA 从细胞骨架框架中释放出来，并且它们的翻译受到抑制。相反，在水泡性口炎病毒感染期间，尽管肌动蛋白合成受到抑制，但肌动蛋白 mRNA 并未从细胞骨架中释放。此外，抑制蛋白质合成的其他几种条件不会导致 mRNA 从细胞骨架框架中释放。我们的结论是，mRNA 与细胞骨架框架的关联是真核生物蛋白质合成所必需的，但还不够。此外，脊髓灰质炎病毒感染期间而非水泡性口炎病毒感染期间宿主蛋白质合成的关闭是通过一种独特的机制发生的，该机制导致宿主mRNA从细胞骨架中释放。
We studied the association of several eucaryotic viral and cellular mRNAs with cytoskeletal fractions derived from normal and virus- infected cells. We found that all mRNAs appear to associate with the cytoskeletal structure during protein synthesis, irrespective of their 5' and 3' terminal structures: e.g., poliovirus that lacks a 5' cap structure or reovirus and histone mRNAs that lack a 3' poly A tail associated with the cytoskeletal framework to the same extent as capped, polyadenylated actin mRNA. Cellular (actin) and viral (vesicular stomatitis virus and reovirus) mRNAs were released from the cytoskeletal framework and their translation was inhibited when cells were infected with poliovirus. In contrast, actin mRNA was not released from the cytoskeleton during vesicular stomatitis virus infection although actin synthesis was inhibited. In addition, several other conditions under which protein synthesis is inhibited did not result in the release of mRNAs from the cytoskeletal framework. We conclude that the association of mRNA with the cytoskeletal framework is required but is not sufficient for protein synthesis in eucaryotes. Furthermore, the shut-off of host protein synthesis during poliovirus infection and not vesicular stomatitis virus infection occurs by a unique mechanism that leads to the release of host mRNAs from the cytoskeleton.
DOI：
10.1016/s0021-9258(18)33352-0
发表时间：
1982-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. Etchison;Susan;Milburn;Isaac Ederys;Nahum Sonenberggl;Hershey
通讯作者：
D. Etchison;Susan;Milburn;Isaac Ederys;Nahum Sonenberggl;Hershey
真核起始因子 4A 参与帽子识别过程。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Edery,I;Hümbelin,M;Darveau,A;Lee,KA;Milburn,S;Hershey,JW;Trachsel,H;Sonenberg,N
通讯作者：
Sonenberg,N