PBC-95K, A 95-KILODALTON NUCLEAR AUTOANTIGEN IN PRIMARY BILIARY-CIRRHOSIS
PBC-95K, A 95-KILODALTON NUCLEAR AUTOANTIGEN IN PRIMARY BILIARY-CIRRHOSIS
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DOI：
10.1002/art.1780340614
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发表时间：
1991-06-01
影响因子：
--
通讯作者：
CRAFT, J
中科院分区：
文献类型：
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作者：
EVANS, J;REUBEN, A;CRAFT, J
我们在原发性胆汁性肝硬化（PBC）患者血清中发现了一种新的抗核抗体（ANA）。 50例PBC患者中有22例的血清在间接免疫荧光上以5-20个离散点的模式染色HEp-2细胞的细胞核。 通常，滴度高于1：1，280。 这种染色模式仅见于其他结缔组织疾病患者的1，240份ANA阳性样本中的4份，而来自49名其他肝病患者的ANA阳性血清中没有一份。 在HeLa细胞核的免疫印迹和S-35-甲硫氨酸标记的HeLa提取物的免疫沉淀物中，将核点染色的血清与95 kd多肽结合。 亲和纯化的抗体从95 kd的蛋白质再现核点免疫荧光染色模式，表明该多肽位于核点。 因此，这种类型的ANA发生在44%的PBC患者中，并且它们似乎对PBC具有特异性。 这些抗体结合一种95 kd的蛋白质，这种蛋白质是离散的，分散在整个细胞核中。
We have identified a novel antinuclear antibody (ANA) in the sera of patients with primary biliary cirrhosis (PBC). Sera from 22 of 50 PBC patients stained the nuclei of HEp-2 cells in a pattern of 5-20 discrete dots, on indirect immunofluorescence. Typically, the titers were higher than 1:1,280. This staining pattern was seen in only 4 of 1,240 ANA-positive samples from patients with other connective tissue diseases, and in none of the ANA-positive sera from 49 patients with other liver diseases. Sera which stained the nuclear dots bound a 95-kd polypeptide in immunoblots of HeLa nuclei and in immunoprecipitates of S-35-methionine-labeled HeLa extracts. Affinity-purified antibodies from the 95-kd protein reproduced the nuclear dot immunofluorescence staining pattern, demonstrating that this polypeptide is located in the nuclear dots. ANA of this type, therefore, occur in 44% of patients with PBC, and they appear to be specific for PBC. These antibodies bind a 95-kd protein that is discrete and is scattered throughout the nucleus.