Fungal antagonists of the plant pathogen Rhizoctonia solani:: selection, control efficacy and influence on the indigenous microbial community
Fungal antagonists of the plant pathogen Rhizoctonia solani:: selection, control efficacy and influence on the indigenous microbial community
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DOI：
10.1016/j.mycres.2006.09.014
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Berg, Gabriele
中科院分区：
文献类型：
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作者：
Grosch, Rita;Scherwinski, Katja;Berg, Gabriele
采用体外和体内结合的新方法，评价了广谱真菌拮抗剂作为土壤源性病原菌索拉根丝核菌的潜在生物防治剂。通过对不同温度条件下菌丝体寄生能力和抑制菌核根丝核菌萌发能力的体外鉴定，筛选出6种潜在的真菌拮抗剂。BOX-PCR指纹图谱对其进行了基因典型鉴定，并通过部分18S rDNA测序鉴定为里氏木霉和绿霉。当马铃薯芽菜用木霉处理时，所有分离株都显著降低了根丝核菌症状的发生率。在生长室条件下进行评价，所选木霉分离株部分或完全控制了番茄枯萎病引起的生菜干质量损失。此外，拮抗木霉菌株在田间条件下也有活性。为了分析木霉处理对本地根相关微生物群落的影响，我们对16S rDNA/ITS序列进行了dna依赖的SSCP（单链构象多态性）分析。本研究首次对木霉进行了评价，结果表明病原菌和植被生长时间对木霉菌群组成的影响远大于BCA处理。在对所有结果进行评估后，从菌核根丝核菌中分离出的3株木霉被选为有希望的bca。(c) 2006年英国真菌学学会。Elsevier Ltd.出版。版权所有。
A broad spectrum of fungal antagonists was evaluated as potential biocontrol agents (BCAs) against the soil-borne pathogen Rhizoctonia solani using a new combination of in vitro and in vivo assays. The in vitro characterisation of diverse parameters including the ability to parasitise mycelium and to inhibit the germination of Rhizoctonia sclerotia at different temperatures resulted in the selection of six potential fungal antagonists. These were genotypically characterised by their BOX-PCR fingerprints, and identified as Trichoderma reesei and T. viride by partial 18S rDNA sequencing. When potato sprouts were treated with Trichoderma, all isolates significantly reduced the incidence of Rhizoctonia symptoms. Evaluated under growth chamber conditions, the selected Trichoderma isolates either partly or completely controlled the dry mass loss of lettuce caused by R. solani. Furthermore, the antagonistic Trichoderma strains were active under field conditions. To analyse the effect of Trichoderma treatment on indigenous root-associated microbial communities, we performed a DNA-dependent SSCP (Single-Strand Conformation Polymorphism) analysis of 16S rDNA/ITS sequences. In this first assessment study for Trichoderma it was shown that the pathogen and the vegetation time had much more influence on the composition of the microbiota than the BCA treatment. After evaluation of all results, three Trichoderma strains originally isolated from Rhizoctonia sclerotia were selected as promising BCAs. (c) 2006 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.