Pituitary basophils from immature male and female rats: distribution of gonadotrophs and thyrotrophs as studied by unit gravity sedimentation.
Pituitary basophils from immature male and female rats: distribution of gonadotrophs and thyrotrophs as studied by unit gravity sedimentation.
复制标题
未成熟雄性和雌性大鼠的垂体嗜碱性粒细胞：通过单位重力沉降研究促性腺激素和促甲状腺激素的分布。
DOI：
10.1210/endo-103-3-724
复制
发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
B. Vanderschueren
中科院分区：
文献类型：
--
作者：
C. Denef;E. Hautekeete;A. de Wolf;B. Vanderschueren
用单位重力沉降法将14日龄雌性、14日龄雄性和成年雄性大鼠的垂体细胞分离成几个部分。对于来自14日龄动物的制剂，嗜碱性粒细胞的百分比从梯度的顶部到底部增加。底部馏分含有大的嗜碱性粒细胞，其纯度在雌性制剂中达到近90%，在雄性制剂中达到75%。从成年雄性大鼠制备的相同组分由超过80%的大嗜酸性粒细胞组成。如免疫染色所示，大多数雌性大嗜碱性粒细胞是促性腺细胞，而在14日龄雄性中，约1/3的嗜碱性粒细胞是促甲状腺细胞。14日龄大鼠中、小型促性腺激素细胞和促甲状腺激素细胞的相对比例也高于成年大鼠。通过比较与抗FSH、抗LH和两种抗血清混合物反应的细胞数，估计FSH和LH在促性腺激素细胞中的分布。这些数据表明，无论细胞大小如何，14日龄大鼠的大多数促性腺激素细胞都含有FSH和LH。在大多数分数中，只有一种激素的细胞的发生在男性比女性更明显。在成年大鼠中，只有FSH的细胞在具有大促性腺激素细胞的部分中最丰富。通过RIA测量的激素含量的分布对于每个动物组具有高度特征。在14日龄的雌性中，超过70%的恢复激素在大型促性腺激素细胞中。在14日龄和成年雄性中，也从中等大小的促性腺激素细胞中回收了大量的激素。目前的研究结果表明垂体激素分泌的发育变化和垂体细胞类型分布的变化之间的关系。
Isolated pituitary cells from 14-day-old female, 14-day-old male, and adult male rats were separated into several fractions by unit gravity sedimentation. With preparations from 14-day-old animals, the percentage of basophils increased from top to bottom of the gradient. The bottom fractions contained large basophils with a purity attaining almost 90% in the female and 75% in the male preparation. The same fraction prepared from adult male rats was composed of over 80% of large acidophils. As identified by immunostaining, the majority of the female large basophils were gonadotrophs, whereas in 14-day-old males about 1/3 of these basophils were thyrotrophs. The relative proportion of medium and small sized gonadotrophs and of thyrotrophs was also higher in the 14-day-old rats than in the adults. An estimate of the distribution of FSH and LH among the gonadotrophs was made by comparing the number of cells reacting with anti-FSH, with anti-LH, and with a mixture of both antisera. The data suggested that irrespective of cell size, the majority of the gonadotrophs in 14-day-old rats contained both FSH and LH. In most fractions, the occurrence of cells with only one hormone was more apparent in the males than in the females. In adult rats, cells with only FSH were most abundant in the fraction with large gonadotrophs. The distribution of hormone content, as measured by RIA, was highly characteristic for each animal group. In 14-day-old females, more than 70% of the recovered hormones was in the large gonadotrophs. In 14-day-old and adult males, substantial amounts of hormone were also recovered from medium sized gonadotrophs. The present findings suggest a relationship between developmental changes in pituitary hormone secretion and changes in pituitary cell type distribution.