Effect of in vitro aspirin stimulation on basophils in patients with aspirin-exacerbated respiratory disease.
Effect of in vitro aspirin stimulation on basophils in patients with aspirin-exacerbated respiratory disease.
复制标题
体外阿司匹林刺激对阿司匹林加重呼吸道疾病患者嗜碱性粒细胞的影响。
DOI：
10.1111/j.1365-2222.2009.03277.x
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Adkinson,NF
中科院分区：
文献类型：
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作者：
Celik,GE;Schroeder,JT;Hamilton,RG;Saini,SS;Adkinson,NF
BackgroundBasophil activation has been implicated in the pathogenesis of aspirin‐exacerbated respiratory disease (AERD). However, a comprehensive analysis of basophil responses to aspirin in terms of mediator release, cytokine secretion and increased expression of surface activation markers has not been performed.ObjectiveTo study thein vitroeffects of aspirin on the concurrent release of histamine, leukotriene C4(LTC4) and IL‐4 from human basophils and to also evaluate changes in surface activation markers (CD63, CD69 and CD203c) expressed by these cells.MethodsBasophil‐enriched cell suspensions from 10 patients with AERD and 10 healthy volunteers were incubated with lysine‐aspirin for up to 3 h. Cells were analysed for expression of CD63, CD69 and CD203c using flow cytometry. Cell‐free supernatants were evaluated for histamine, and LTC4release and for IL‐4 secretion.ResultsAspirin‐induced expression of CD63, CD69 and CD203c yielded 30%, 80% and 70% sensitivity, respectively, but with poor specificity. There was no significant difference in LTC4synthesis between groups. None of the patients with AERD (or controls) released IL‐4 in response to aspirin. A higher dose of 5 mg/mL aspirin‐mediated non‐specific effects on basophils.ConclusionBasophil responses toin vitroaspirin challenge are poor indicators of clinical sensitivity. Aspirin activates some basophils by means of mechanisms that differ from the classical IgE‐mediated pathway. Our study also shows that the use of 27 mmof aspirin (5 mg/mL) by previous investigators causes non‐specific basophil activation, thereby eliminating its usefulness in a cell‐based diagnostic test for AERD. Evaluation ofin vitrobasophil activation has low clinical value in identifying aspirin‐induced respiratory reactions.
