Interleukin-4 induced IgE and IgG4 secretion by B cells from atopic dermatitis patients.
Interleukin-4 induced IgE and IgG4 secretion by B cells from atopic dermatitis patients.
复制标题
Interleukin-4 诱导特应性皮炎患者 B 细胞分泌 IgE 和 IgG4。
DOI：
10.1159/000235357
复制
发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Beck,L
中科院分区：
文献类型：
--
作者：
Spiegelberg,HL;O'Connor,RD;Falkoff,RJ;Beck,L
用重组白细胞介素-4（IL-4）培养8例正常人和8例特应性皮炎（AD）患者外周血单个核细胞，用放射免疫法测定培养上清中IgE和IgG亚类水平。IL-4诱导正常人和AD患者B细胞分泌IgE和IgG 4，而对IgG 1、IgG 2和IgG 3的分泌无一致性影响。正常人和AD患者细胞分泌IgE和IgG 4的IL-4剂量反应相似。平均而言，患者的细胞比正常人分泌更多的IgE和更少的IgG 4，但由于差异很大，差异并不显著。然而，正常人的IgG 4：IgE分泌比率显著大于AD患者（平均值± SEM 7.1 ± 1.6：1对比1.5 ± 0.4：1; p < 0.01）。数据表明，IL-4诱导来自正常人和AD患者的B细胞的IgE和IgG 4分泌，并表明与正常人相比，在AD患者中IL-4诱导的从IgM到IgG 4或IgE分泌的转换可能优先于IgE进行。
Peripheral blood mononuclear cells from 8 normals and 8 patients with atopic dermatitis (AD) were cultured with recombinant interleukin-4 (IL-4) and the IgE and IgG subclass levels in the culture supernatants measured by radioimmunoassays. IL-4 induced IgE and IgG4 secretion by B cells from both normals and AD patients whereas it has no consistent effect on IgG1, IgG2 and IgG3 secretion. The IL-4 dose response was similar for IgE and IgG4 secretion by cells from both normals and AD patients. On the average, the patients’ cells secreted more IgE and less IgG4 than the cells from normals, but because of a large variation, the differences were not significant. However, the ratio of IgG4:IgE secretion was significantly greater for normals than AD patients (mean ± SEM 7.1 ± 1.6:1 vs. 1.5 ± 0.4:1; p < 0.01). The data demonstrate that IL-4 induces IgE and IgG4 secretion by B cells from both normals and AD patients and suggest that the IL-4 induced switch from IgM to IgG4 or IgE secretion may proceed preferentially to IgE in AD patients as compared to normals.