"Doubly Selective" Antimicrobial Polymers: How Do They Differentiate between Bacteria?
"Doubly Selective" Antimicrobial Polymers: How Do They Differentiate between Bacteria?
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DOI：
10.1002/chem.200802558
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Tew, Gregory N.
中科院分区：
文献类型：
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作者：
Lienkamp, Karen;Kumar, Kushi-Nidhi;Tew, Gregory N.
我们已经研究了抗菌肽的双重选择性合成模拟物（SMAMPs）是如何区分革兰氏阴性菌和革兰氏阳性菌的，SMAMPs不仅可以区分细菌和哺乳动物细胞，而且可以区分革兰氏阴性菌和革兰氏阳性菌。通过对模型囊泡的染料渗漏实验和补充实验，我们能够将谷粒选择性与这些细菌类型的结构差异联系起来。结果表明，E.而不是E. coli和革兰氏阳性菌S.金黄色葡萄球菌对革兰氏阳性菌的选择性敏感。SMAMP的分子量依赖性抗菌活性表现为筛选效应：而3000 g mol（-1）SMAMP能够穿透革兰氏阳性菌的肽聚糖层。金黄色葡萄球菌，5000 gmol-（1）SMAMP被粘住，因此不具有抗微生物活性。
We have investigated how doubly selective synthetic mimics of antimicrobial peptides (SMAMPs), which can differentiate not only between bacteria and mammalian cells, but also between Gram-negative and Gram-positive bacteria, make the latter distinction. By dye-leakage experiments on model vesicles and complementary experiments oil bacteria, we were able to relate the Grain selectivi-ty to structural differences of these bacteria types. We showed that the double membrane of E. coli rather than the difference in lipid composition between E. coli and S. aureus was rasponsible for Gram selectivity. The molecular-weight-dependent antimicrobial activity of the SMAMPs was shown to be a sieving effect: while the 3000 g mol(-1) SMAMP was able to penetrate the peptidoglycan layer of the Gram-positive S. aureus bacteria, the 5000 gmol-(1) SMAMP got stuck and consequently did not have antimicrobial activity.