NUCLEOTIDE-SEQUENCE OF RIGHTWARD OPERATOR OF PHAGE LAMBDA
NUCLEOTIDE-SEQUENCE OF RIGHTWARD OPERATOR OF PHAGE LAMBDA
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DOI：
10.1073/pnas.72.3.1184
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发表时间：
1975-01-01
影响因子：
11.1
通讯作者：
KLEID, DG
中科院分区：
文献类型：
--
作者：
MANIATIS, T;JEFFREY, A;KLEID, DG
本文报道了λ噬菌体操纵子（O-R）的72个碱基对的序列，用简单快速的DNA直接测序方法测定了其序列。还描述了操纵基因突变体的序列。该方法通常用于对具有独特的5'端的长度高达50个碱基对的DNA片段进行测序。先前的实验已经表明，该操纵子包含λ噬菌体阻遏物识别的多个位点。我们相信我们已经确定了其中三个地点。
The sequence of 72 base pairs of the rightward operator (O-R) of bacteriophage lambda is presented as determined with simple and rapid methods for direct DNA sequencing. The sequence of an operator mutant is also described. The methods are of general use in sequencing DNA fragments with unique 5' ends up to 50 base pairs in length. Previous experiments have shown that this operator contains multiple sites recognized by the lambda phage repressor. We believe we have identified three of these sites.