Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode.
Direct electrochemistry and electrocatalytic activity of cytochrome c covalently immobilized on a boron-doped nanocrystalline diamond electrode.
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DOI：
10.1021/ac702417x
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发表时间：
2008-04
影响因子：
7.4
通讯作者：
Yanli Zhou;Jinfang Zhi;Y. Zou;Wenjun Zhang;Shui-Tong Lee
中科院分区：
文献类型：
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作者：
Yanli Zhou;Jinfang Zhi;Y. Zou;Wenjun Zhang;Shui-Tong Lee
将细胞色素c（Cyt c）共价固定在硼掺杂纳米金刚石（BDND）电极上，通过十一碳烯酸甲酯的表面功能化和随后去除保护酯基团以产生羧基封端的表面。在0.1M磷酸盐缓冲溶液（pH7.0）中，Cyt c修饰的BDNF电极呈现一对准可逆的氧化还原峰，其形式电位（E（0））为0.061V（vsAg/AgCl），电子转移常数（ks）为5.2 ± 0.6s（-1），为表面控制过程。并对沉积态和Cyt c修饰的掺硼微晶金刚石（BDMD）电极的电化学性能进行了比较。对过氧化氢（H2 O2）的细胞色素c修饰的BDNF电极的电催化活性的调查显示，快速的安培响应（5秒）。响应H2 O2浓度的线性范围为1至450 μ M，在信噪比为3时，检测限为0.7 μ M。与BDMD和玻碳电极相比，Cyt c修饰的BDNF电极的稳定性也进行了评价。
Cytochrome c (Cyt c) was covalently immobilized on a boron-doped nanocrystalline diamond (BDND) electrode via surface functionalization with undecylenic acid methyl ester and subsequent removal of the protecting ester groups to produce a carboxyl-terminated surface. Cyt c-modified BDND electrode exhibited a pair of quasi-reversible and well-defined redox peaks with a formal potential (E(0)) of 0.061 V (vs Ag/AgCl) in 0.1 M phosphate buffer solution (pH 7.0) and a surface-controlled process with a high electron transfer constant (ks) of 5.2 +/- 0.6 s(-1). The electrochemical properties of as-deposited and Cyt c-modified boron-doped microcrystalline diamond (BDMD) electrodes were also studied for comparison. Investigation of the electrocatalytic activity of the Cyt c-modified BDND electrode toward hydrogen peroxide (H2O2) revealed a rapid amperometric response (5 s). The linear range of response to H2O2 concentration was from 1 to 450 microM, and the detection limit was 0.7 microM at a signal-to-noise ratio of 3. The stability of the Cyt c-modified BDND electrode, in comparison with that of the BDMD and glassy carbon counterpart electrodes, was also evaluated.