Unique biomarkers for B-cell function predict the serum response to pandemic H1N1 influenza vaccine
Unique biomarkers for B-cell function predict the serum response to pandemic H1N1 influenza vaccine
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DOI：
10.1093/intimm/dxr123
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发表时间：
2012-03-01
影响因子：
4.4
通讯作者：
Blomberg, Bonnie B.
中科院分区：
文献类型：
--
作者：
Frasca, Daniela;Diaz, Alain;Blomberg, Bonnie B.
为了开发有益的体液免疫应答的预测标志物，我们评估了年轻人和老年人对大流行性（p）H1N1疫苗的体内和体外应答。我们测量了血清抗体反应，并将其与体外B细胞对疫苗的反应（通过激活诱导的胞苷脱氨酶（AID）测量）相关联。这两种反应都随着年龄的增长而下降，并且显着相关。在接种疫苗前后，血液中转换记忆B细胞的百分比随着年龄的增长而下降。转换记忆B细胞的百分比在t0与血凝抑制反应，因此，我们建议，这可能被用作B细胞反应性的预测标志物。在接种疫苗之前由CpG诱导的AID也预测疫苗应答的稳健性。仅在年轻个体中接种疫苗后，浆母细胞显示出增加的趋势。本报告建立了应答的分子生物标志物，转换记忆B细胞的百分比和对CpG的AID应答，可用于识别应答不良风险的个体，也可用于测量疫苗的改善和监测最佳体液应答。
In order to develop predictive markers for a beneficial humoral immune response, we evaluated the in vivo and in vitro response to the pandemic (p)H1N1 vaccine in young and elderly individuals. We measured serum antibody response and associated this with the in vitro B-cell response to the vaccine, measured by activation-induced cytidine deaminase (AID). Both responses decrease with age and are significantly correlated. The percentage of switched memory B cells in blood, both before and after vaccination, is decreased with age. The percentage of switched memory B cells at t0 correlates with the hemagglutination inhibition response and therefore, we suggest that this may be used as a predictive marker for B-cell responsiveness. AID induced by CpG before vaccination also predicts the robustness of the vaccine response. Plasmablasts showed a trend to increase after vaccination in young individuals only. This report establishes molecular biomarkers of response, percentage of switched memory B cells and AID response to CpG, useful for identifying individuals at risk of poor response and also for measuring improvements in vaccines and monitoring optimal humoral responses.