Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VII. RNA polymerase is a target for ppGpp.
Genetic studies on the beta subunit of Escherichia coli RNA polymerase. VII. RNA polymerase is a target for ppGpp.
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大肠杆菌RNA聚合酶β亚基的遗传学研究。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
A. Ishihama
中科院分区：
文献类型：
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作者：
R. Glass;S. Jones;A. Ishihama
RNA 聚合酶是从两种抑制 rpoB 的琥珀菌株中分离出来的，对体内 RNA 合成表现出宽松的控制。对这些在 β 亚基已知位点携带确定取代的突变全酶的体外转录分析清楚地表明，β 中的单个氨基酸变化使 RNA 聚合酶对 ppGpp 具有抗性。这明确表明 RNA 聚合酶确实是 ppGpp 的靶标。
RNA polymerase was isolated from two suppressed rpoB amber strains exhibiting relaxed control over RNA synthesis in vivo. In vitro transcription analysis of these mutant holoenzymes carrying defined substitutions at known sites in the beta subunit clearly shows that single amino acid changes in beta render RNA polymerase resistant to ppGpp. This unambiguously demonstrates that RNA polymerase is indeed the target for ppGpp.