Evidence of SARS-CoV-2 Transcriptional Activity in Cardiomyocytes of COVID-19 Patients without Clinical Signs of Cardiac Involvement
Evidence of SARS-CoV-2 Transcriptional Activity in Cardiomyocytes of COVID-19 Patients without Clinical Signs of Cardiac Involvement
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DOI：
10.3390/biomedicines8120626
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发表时间：
2020-12-18
期刊：
影响因子：
4.7
通讯作者：
Pompilio G
中科院分区：
文献类型：
--
作者：
Bulfamante GP;Perrucci GL;Falleni M;Sommariva E;Tosi D;Martinelli C;Songia P;Poggio P;Carugo S;Pompilio G
目的：相当一部分受冠状病毒呼吸道疾病（COVID-19）影响的患者会发生心脏损伤。然而，病毒对心肌细胞的影响值得进一步研究，特别是在无症状患者中。研究方法：我们调查了连续六名死于呼吸衰竭的COVID-19患者的心脏组织中SARS-CoV-2的存在和活性，这些患者没有心脏受累的迹象，也没有心脏病史。在死亡后2 h内收集心脏尸检样本，然后通过数字PCR、Western印迹、免疫组织化学、免疫荧光、RNAScope和透射电子显微镜分析进行分析。结果：在所有实验中均检测到心肌细胞中存在SARS-CoV-2。观察到心肌细胞损伤的可变模式，从细胞死亡和亚细胞改变标志的缺失到细胞内水肿和肌节破裂。此外，通过检测SARS-CoV-2的正、反义刺突RNA，我们发现心肌细胞中病毒转录活跃。结论：在没有心脏受累临床体征的患者的尸检分析中，检测到心肌细胞中存在SARS-CoV-2，确定了细胞内损伤的可变模式。这些发现表明，需要对未显示心脏表型的存活COVID-19患者进行心脏病学监测。
Aims: A considerable proportion of patients affected by coronavirus respiratory disease (COVID-19) develop cardiac injury. The viral impact in cardiomyocytes deserves, however, further investigations, especially in asymptomatic patients. Methods: We investigated for SARS-CoV-2 presence and activity in heart tissues of six consecutive COVID-19 patients deceased from respiratory failure showing no signs of cardiac involvement and with no history of heart disease. Cardiac autopsy samples were collected within 2 h after death, and then analysed by digital PCR, Western blot, immunohistochemistry, immunofluorescence, RNAScope, and transmission electron microscopy assays. Results: The presence of SARS-CoV-2 into cardiomyocytes was invariably detected in all assays. A variable pattern of cardiomyocyte injury was observed, spanning from absence of cell death and subcellular alterations hallmarks, to intracellular oedema and sarcomere ruptures. In addition, we found active viral transcription in cardiomyocytes, by detecting both sense and antisense SARS-CoV-2 spike RNA. Conclusions: In this autopsy analysis of patients with no clinical signs of cardiac involvement, the presence of SARS-CoV-2 in cardiomyocytes has been detected, determining variable patterns of intracellular damage. These findings suggest the need for cardiologic surveillance in surviving COVID-19 patients not displaying a cardiac phenotype.
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影响因子：
3.5
作者：
Fineschi V;Aprile A;Aquila I;Arcangeli M;Asmundo A;Bacci M;Cingolani M;Cipolloni L;D'Errico S;De Casamassimi I;Di Mizio G;Di Paolo M;Focardi M;Frati P;Gabbrielli M;La Russa R;Maiese A;Manetti F;Martelloni M;Mazzeo E;Montana A;Neri M;Padovano M;Pinchi V;Pomara C;Ricci P;Salerno M;Santurro A;Scopetti M;Testi R;Turillazzi E;Vacchiano G;Scientific Society of Hospital Legal Medicine of the National Health System (COMLAS);Crivelli F;Bonoldi E;Facchetti F;Nebuloni M;Sapino A;Italian Society of Anatomical Pathology and Cytology (SIAPEC)
通讯作者：
Italian Society of Anatomical Pathology and Cytology (SIAPEC)
DOI：
10.1152/ajpheart.00426.2008
发表时间：
2008-12-01
影响因子：
4.8
作者：
Gallagher, Patricia E.;Ferrario, Carlos M.;Tallant, E. Ann
通讯作者：
Tallant, E. Ann
影响因子：
4.1
作者：
Wang, Fay;Flanagan, John;Luo, Yuling
通讯作者：
Luo, Yuling
影响因子：
24
作者：
Inciardi, Riccardo M.;Lupi, Laura;Metra, Marco
通讯作者：
Metra, Marco
影响因子：
3.8
作者：
Escher, Felicitas;Pietsch, Heiko;Schultheiss, Heinz-Peter
通讯作者：
Schultheiss, Heinz-Peter