Stereochemistry of 1-(4'-hydroxyphenyl)ethanol produced by hydroxylation of 4-ethylphenol by p-cresol methylhydroxylase.
Stereochemistry of 1-(4'-hydroxyphenyl)ethanol produced by hydroxylation of 4-ethylphenol by p-cresol methylhydroxylase.
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对甲酚甲基羟化酶将 4-乙基苯酚羟基化产生 1-(4-羟基苯基)乙醇的立体化学。
DOI：
10.1042/bj2240617
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Singer,TP
中科院分区：
文献类型：
--
作者：
McIntire,W;Hopper,DJ;Craig,JC;Everhart,ET;Webster,RV;Causer,MJ;Singer,TP
恶臭假单胞菌（a）和高纯度对甲酚甲基羟化酶（B）催化4-乙基苯酚的羟化反应，得到光学活性的1-（4 '-羟基苯基）-乙醇。产物转化为酚甲基醚，分别含有69.5%和65.6%的（S）-（-）-异构体。的反应的立体化学进行了讨论的三个不同的步骤发生在酶的活性位点。
Enzymic hydroxylation of 4-ethylphenol by (a) Pseudomonas putida and (b) highly purified p-cresol methylhydroxylase gave optically active 1-(4'-hydroxyphenyl)-ethanol. The products were transformed into the phenolic methyl ethers and shown to contain 69.5% and 65.6%, respectively, of the (S)-(-)-isomer. The stereochemistry of the reaction is discussed in terms of three distinct steps occurring at the active site of the enzyme.