Dopamine neurons release transmitter via a flickering fusion pore
Dopamine neurons release transmitter via a flickering fusion pore
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DOI：
10.1038/nn1205
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发表时间：
2004-04-01
影响因子：
25
通讯作者：
Sulzer, D
中科院分区：
文献类型：
--
作者：
Staal, RGW;Mosharov, EV;Sulzer, D
了解神经递质释放机制的一个关键问题是突触小泡的融合孔是否调节胞吐过程中释放的递质量。我们使用碳纤维电流分析法测量了大鼠培养的腹侧中脑神经元小突触囊泡的多巴胺释放。我们的数据表明，小突触囊泡融合孔快速连续闪烁一次或多次，每次闪烁释放类似于 25-30% 的囊泡多巴胺。佛波酯和十字孢菌素相互调节多重闪烁事件的发生率。因此，多巴胺神经元通过控制每个胞吐事件的融合孔闪烁数量来调节小突触小泡释放的神经递质的量。这种胞吐作用模式是一种潜在机制，神经元可以快速重复利用囊泡，而无需经历相对缓慢的回收过程。
A key question in understanding mechanisms of neurotransmitter release is whether the fusion pore of a synaptic vesicle regulates the amount of transmitter released during exocytosis. We measured dopamine release from small synaptic vesicles of rat cultured ventral midbrain neurons using carbon fiber amperometry. Our data indicate that small synaptic vesicle fusion pores flicker either once or multiple times in rapid succession, with each flicker releasing similar to25-30% of vesicular dopamine. The incidence of events with multiple flickers was reciprocally regulated by phorbol esters and staurosporine. Thus, dopamine neurons regulate the amount of neurotransmitter released by small synaptic vesicles by controlling the number of fusion pore flickers per exocytotic event. This mode of exocytosis is a potential mechanism whereby neurons can rapidly reuse vesicles without undergoing the comparatively slow process of recycling.