Discovery of a novel superfamily of type III polyketide synthases in Aspergillus oryzae
Discovery of a novel superfamily of type III polyketide synthases in Aspergillus oryzae
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DOI：
10.1016/j.bbrc.2005.03.160
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发表时间：
2005-05-27
影响因子：
3.1
通讯作者：
Kitamoto, K
中科院分区：
文献类型：
--
作者：
Seshime, Y;Juvvadi, PR;Kitamoto, K
在工业上有用的丝状真菌，Aspergillusspirum中，编码III型聚酮合酶（PKS）超家族成员的基因的鉴定显示，它们的分布不是植物或细菌所特有的。在其它曲霉属（构巢曲霉和烟曲霉）中，A.该基因具有4个查尔酮合酶（CHS）样基因（csyA、csyB、csyC和csyD）。csyA的表达。通过RT-PCR证实了esyB和csyD基因的存在。比较基因组分析显示，在粗糙脉孢菌和禾谷镰刀菌中有一个推定的III型PKS，在稻瘟病菌和鹅柄孢菌中各有两个，在Phenarocheate chrysosporium中有三个，与细菌和植物有明显的区别。在跨物种的CHSs中的催化残基的保护暗示这些新发现的同系物的酶活性性质。(c)2005年爱思唯尔公司All rights reserved.
Identification of genes encoding type III polyketide synthase (PKS) superfamily members in the industrially useful filamentous fungus, Aspergillus oryzae, revealed that their distribution is not specific to plants or bacteria. Among other Aspergilli (Aspergillus nidulans and Aspergillus fumigatus), A. oryzae was unique in possessing four chalcone synthase (CHS)-like genes (csyA, csyB, csyC, and csyD). Expression of csyA. esyB, and csyD genes was confirmed by RT-PCR. Comparative genome analyses revealed single putative type III PKS in Neurospora crassa and Fusarium graminearum, two each in Magnaporthe grisea and Podospora anserina, and three in Phenarocheate chrysosporium, with a phylogenic distinction from bacteria and plants. Conservation of catalytic residues in the CHSs across species implicated enzymatically active nature of these newly discovered homologs. (c) 2005 Elsevier Inc. All rights reserved.