DIMER FORM OF PHOSPHORYLATED SPO0A, A TRANSCRIPTIONAL REGULATOR, STIMULATES THE SPO0F TRANSCRIPTION AT THE INITIATION OF SPORULATION IN BACILLUS-SUBTILIS
DIMER FORM OF PHOSPHORYLATED SPO0A, A TRANSCRIPTIONAL REGULATOR, STIMULATES THE SPO0F TRANSCRIPTION AT THE INITIATION OF SPORULATION IN BACILLUS-SUBTILIS
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DOI：
10.1006/jmbi.1995.0354
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发表时间：
1995-06-30
影响因子：
5.6
通讯作者：
KOBAYASHI, Y
中科院分区：
文献类型：
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作者：
ASAYAMA, M;YAMAMOTO, A;KOBAYASHI, Y
枯草芽孢杆菌的SpoOA蛋白是一种转录调节因子，与细菌双组分调节系统中的调节蛋白具有广泛的同源性。SpoOA的磷酸化对于孢子形成的起始是绝对必要的。我们现在表明，磷酸化SpoOA是一个二聚体，特异性地结合到spoOF启动子区域，并刺激从西格玛（H）-RNA聚合酶识别的P2启动子的转录。生化和生物学分析表明，磷酸化SpoOA直接与位于spoOF启动子区域的“OA样盒”序列（TGTCGTA）相互作用。SpoOA的磷酸化增强了其对OA样盒的亲和力。还提出的证据表明，spoOF启动子区域包含一个静态的弯曲具有两套寡（dA-dT）tracts。证明了弯曲区域与磷酸化SpoOA的识别位点重叠。
The SpoOA protein of Bacillus subtilis is a transcriptional regulator that shows extensive homology to the regulator proteins in bacterial two-component regulatory systems. Phosphorylation of SpoOA is absolutely necessary for the initiation of sporulation. We now show that phospho-SpoOA is a dimer, binds specifically to the spoOF promoter region, and stimulates the transcription from the P2 promoter recognized by sigma(H)-RNA polymerase. Biochemical and biological analyses suggest that phospho-SpoOA interacts directly with the ''OA-like box'' sequence (TGTCGTA) located in the spoOF promoter region. Phosphorylation of SpoOA enhanced its affinity to the OA-like box. Evidence is also presented that the spoOF promoter region contains a static bend having two sets of oligo(dA-dT) tracts. It was demonstrated that the bending region overlaps with the recognition site for the phospho-SpoOA.