Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype
Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype
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DOI：
10.1007/s00709-010-0162-4
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发表时间：
2011-04-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Yuejin
中科院分区：
文献类型：
--
作者：
Guan, Xin;Zhao, Heqing;Wang, Yuejin
葡萄属假网状葡萄乙二醛氧化酶（VpGLOX）先前在筛选响应于葡萄藤白粉病（Erysiphe necator，PM）感染而上调的基因期间从中国野生葡萄假网状葡萄登记号“百合-35-1”中分离。本研究利用农杆菌介导的瞬时表达技术研究VpGLOX基因在植物抗PM中的可能功能。在优化农杆菌渗入后，VpGLOX在PM敏感（登记“6-12-2”）或PM抗性（登记“6-12-6”）植物的叶中瞬时过表达。使用β-葡萄糖醛酸酶（GUS）报告基因验证转染效率，并且发现其包括大多数叶面积，而与初始叶位置无关。感染E. necator的，明显的差异，观察到之间的农渗透叶和对照组，敏感和抗性，基因型的菌丝发育。VpGLOX的表达，其次是在两种基因型的实时聚合酶链反应。而在易感宿主（“6-12-2”）中，发现表达仅在转染的叶中增加并保持瞬时，在抗性宿主（“6-12-6”）中，第二个峰稍后出现在转染的叶中，可能代表内源VpGLOX的响应。数据支持VpGLOX足以赋予对E.钩虫
Vitis pseudoreticulata glyoxal oxidase (VpGLOX) was previously isolated from the Chinese wild vine V. pseudoreticulata accession "Baihe-35-1" during a screen for genes that are upregulated in response to infection with grapevine powdery mildew (Erysiphe necator, PM). In the present study, a possible function of VpGLOX for defense against PM was investigated using Agrobacterium-mediated transient expression. After optimizing agro-infiltration, VpGLOX was transiently overexpressed in leaves of either PM-susceptible (accession "6-12-2") or PM-resistant (accession "6-12-6") plants. The efficiency of transfection was verified using a beta-glucuronidase (GUS) reporter and was found to comprise most leaf areas regardless of the initial leaf position. Upon infection with E. necator, clear differences were observed with respect to hyphal development between agro-infiltrated leaves and control groups of both, the susceptible and the resistant, genotypes. The expression of VpGLOX was followed by real-time polymerase chain reaction in both genotypes. Whereas in the susceptible host ("6-12-2") expression was found to increase only in transfected leaves and remained transient, in the resistant host ("6-12-6"), a second peak appeared later in transfected leaves, probably representing the response of the endogenous VpGLOX. The data support the interpretation that VpGLOX is sufficient to confer resistance to E. necator.