Sensory experience and sensory activity regulate chemosensory receptor gene expression in Caenorhabditis elegans
Sensory experience and sensory activity regulate chemosensory receptor gene expression in Caenorhabditis elegans
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DOI：
10.1073/pnas.191352498
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发表时间：
2001-09-25
影响因子：
11.1
通讯作者：
Bargmann, CI
中科院分区：
文献类型：
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作者：
Peckol, EL;Troemel, ER;Bargmann, CI
环境的变化会引起动物行为的短期和长期变化。在这里，我们表明，特定的感觉经验导致化学感受器基因表达的变化，可能会改变线虫的感觉。三个预测的化学感受器基因表达的ASI化学感受神经元，srd-1，str-2和str-3，被压抑暴露于dauer信息素，拥挤的信号。抑制发生在信息素浓度低于那些诱导形成的替代dauer幼虫阶段，表明暴露于信息素可以改变非dauer动物的化学感觉行为。此外，ASI表达的srd-1，而不是str-2和str-3，是由ASI神经元的感觉活动。两个受体基因的表达受发育进入dauer幼虫阶段的调控。SRD-1在ASI神经元中的表达在Dauer幼虫中被抑制。在成年动物中，str-2的表达在ASI神经元中被诱导，但在AWC神经元中被抑制。ASI和AWC神经元在Dauer期发生重塑，这些结果表明它们的感觉特异性也发生了变化。我们认为化学感受器基因表达的经验依赖性变化可能会改变嗅觉行为。
Changes in the environment cause both short-term and long-term changes in an animal's behavior. Here we show that specific sensory experiences cause changes in chemosensory receptor gene expression that may alter sensory perception in the nematode Caenorhabditis elegans. Three predicted chemosensory receptor genes expressed in the ASI chemosensory neurons, srd-1, str-2, and str-3, are repressed by exposure to the dauer pheromone, a signal of crowding. Repression occurs at pheromone concentrations below those that induce formation of the alternative dauer larva stage, suggesting that exposure to pheromones can alter the chemosensory behaviors of non-dauer animals. In addition, ASI expression of srd-1, but not str-2 and str-3, is induced by sensory activity of the ASI neurons. Expression of two receptor genes is regulated by developmental entry into the dauer larva stage. srd-1 expression in ASI neurons is repressed in dauer larvae. str-2 expression in dauer animals is induced in the ASI neurons, but repressed in the AWC neurons. The ASI and AWC neurons remodel in the dauer stage, and these results suggest that their sensory specificity changes as well. We suggest that experience-dependent changes in chemosensory receptor gene expression may modify olfactory behaviors.