Chemical defence by mono-prenyl hydroquinone in a freshwater ciliate, Spirostomum ambiguum
Chemical defence by mono-prenyl hydroquinone in a freshwater ciliate, Spirostomum ambiguum
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DOI：
10.1007/s10750-011-0972-1
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发表时间：
2012-03-01
期刊：
影响因子：
2.6
通讯作者：
Ortenzi, Claudio
中科院分区：
文献类型：
--
作者：
Buonanno, Federico;Guella, Graziano;Ortenzi, Claudio
几种纤毛虫在特殊的膜结合可喷射细胞器：挤出体中产生并积累低分子量有毒化合物。 These compounds can be used in predator-prey interaction for killing prey as well as for chemical defence. Here, we describe the isolation and characterisation of 2-(3-methylbut-2-enyl)benzene-1,4-diol(mono-prenyl hydroquinone), the extrusomal defensive toxin of the freshwater heterotrich ciliate Spirostomum ambiguum. The toxin was purified at homogeneity by RP-HPLC, and its structural characterisation was carried out through NMR and MS measurements. In vivo experiments involving S. ambiguum and Climacostomum virens in predator-prey interaction, and the analysis of cytotoxic activity of mono-prenyl hydroquinone on a panel of free-living freshwater ciliates, indicated that the toxin is very effective in S. ambiguum's chemical defence.
Several species of ciliates produce and accumulate low molecular weight toxic compounds in specialised membrane-bound ejectable organelles: extrusomes. These compounds can be used in predator-prey interaction for killing prey as well as for chemical defence. Here, we describe the isolation and characterisation of 2-(3-methylbut-2-enyl)benzene-1,4-diol(mono-prenyl hydroquinone), the extrusomal defensive toxin of the freshwater heterotrich ciliate Spirostomum ambiguum. The toxin was purified at homogeneity by RP-HPLC, and its structural characterisation was carried out through NMR and MS measurements. In vivo experiments involving S. ambiguum and Climacostomum virens in predator-prey interaction, and the analysis of cytotoxic activity of mono-prenyl hydroquinone on a panel of free-living freshwater ciliates, indicated that the toxin is very effective in S. ambiguum's chemical defence.