Gap-junctional communication compartments in the Drosophila wing imaginal disk.
Gap-junctional communication compartments in the Drosophila wing imaginal disk.
复制标题
果蝇翼成虫盘中的间隙连接通讯室。
DOI：
10.1016/0012-1606(84)90181-7
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
Lo,CW
中科院分区：
文献类型：
--
作者：
Weir,MP;Lo,CW
通过细胞内注射荧光小分子，我们研究了果蝇翅膀想象盘细胞的缝隙连接通讯特性。我们观察到注射的染料在翼盘上皮各部分的细胞之间广泛扩散，但我们发现这种扩散并不均匀。取而代之的是，染料在细胞之间的移动在几个定义的边界上被部分地限制，使得边界一侧的细胞与另一侧的细胞之间的通信水平较低。这些通讯限制的边界由通讯水平较低的细胞带所划定，它们将盘上皮细分为若干个“通讯间隔”。有趣的是，其中一些通信间隔的边界似乎与细胞谱系研究中确定的间隔边界一致。鉴于这一惊人的巧合，我们认为缝隙连接通讯可能在隔室的形成和控制隔室内细胞的适当分化方面发挥作用。
Using intracellular injection of small fluorescent molecules, we examined the gap-junctional communication properties of cells in the wing imaginal disk ofDrosophila. We observed extensive spread of the injected dye between cells in all parts of the wing disk epithelium, but we found that this spread is not uniform. Instead, the cell-to-cell movement of dye is partially restricted at several defined boundaries such that cells on one side of the boundary have a low level of communication with cells on the other side. These communication-restriction boundaries are delineated by bands of cells with a low level of communication, and they subdivide the disk epithelium into a number of “communication compartments.” Interestingly, the boundaries of some of these communication compartments appear to be coincident with compartment borders identified in cell lineage studies. Given this striking coincidence, we suggest that gap-junctional communication may play a role in compartment formation, and in controlling the appropriate differentiation of cells within compartments.