Motility and Chemotaxis
Motility and Chemotaxis
复制标题
运动性和趋化性
DOI：
10.1128/9781555818388.ch53
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Chamberlin
中科院分区：
文献类型：
--
作者：
G. Ordal;L. Marquez;M. Chamberlin
本章回顾了fla-che-mot基因的遗传学和作图，然后是运动和趋化性的机制，最后是fla-che-mot系统中基因表达的调控。它还集中在一些重要的突变类别。根据其降低自溶酶表达水平的能力，已经分离出几种突变。这些菌株通过最小诱变获得，发现含有产生Lyt-弗拉-表型的单一突变。肠道细菌中的趋化性是通过结合特异性引诱剂的细胞受体来介导的。这种结合启动了一个信号，该信号通过特异性甲基化-去甲基化和磷酸化-去磷酸化通过一系列蛋白质中间体转导。关于感觉转导机制的最详细信息来自对肠道细菌的研究，其中涉及结构和调节作用的大多数基因可能是已知的，并且已经测序。与大肠杆菌一样，枯草芽孢杆菌的甲基化趋化蛋白（MCP）是一种整合的膜蛋白，由于S-腺苷甲硫氨酸的甲基转移，在谷氨酸侧链上发生甲酯化。感觉通路基因的表达在两个B中都受到调节。通过生长培养基的组成、细胞生长的阶段以及通过功能性感觉系统所需的其它fla-che-mot基因的表达来区分枯草杆菌和肠细菌。
This chapter reviews the genetics and mapping of fla-che-mot genes, then the mechanisms of motility and chemotaxis, and finally, what is known about regulation of gene expression in the fla-che-mot system. It also concentrates on some important classes of mutations. Several mutations have been isolated on the basis of their abilities to reduce the level of autolytic enzyme expression. These strains were obtained by minimal mutagenesis and found to contain a single mutation that gave rise to a Lyt- Fla- phenotype. Chemotaxis in enteric bacteria is mediated through sets of cellular receptors that bind specific attractants. Such binding initiates a signal that is transduced through a series of protein intermediates by specific methylation-demethylation and phosphorylation-dephosphorylation. The most detailed information about the mechanisms involved in sensory transduction comes from studies of enteric bacteria, in which the majority of the genes involved in structural and regulatory roles are probably known and have been sequenced. As in Escherichia coli, the methylated chemotaxis proteins (MCPs) of Bacillus subtilis are integral membrane proteins that are methyl esterified on glutamate side chains as the result of methyl transfer from S-adenosylmethionine. Expression of the genes of the sensory pathway is regulated in both B. subtilis and enteric bacteria by the composition of the growth medium, by the stage of cell growth, and by the expression of other fla-che-mot genes needed for a functional sensory system.
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枯草芽孢杆菌鞭毛钩-基体复合体中的基因-蛋白质关系：flgB、flgC、flgG、fliE 和 fliF 基因的序列。
影响因子：
3.5
作者：
Zuberi,AR;Ying,C;Bischoff,DS;Ordal,GW
通讯作者：
Ordal,GW
枯草芽孢杆菌中引诱剂诱导的甲基接受趋化蛋白甲基化的快速变化。
影响因子：
2.9
作者：
Thoelke,MS;Parker,HM;Ordal,EA;Ordal,GW
通讯作者：
Ordal,GW
DOI：
10.1073/pnas.87.1.41
发表时间：
1990-01-01
影响因子：
11.1
作者：
BOURRET, RB;HESS, JF;SIMON, MI
通讯作者：
SIMON, MI
DOI：
10.1016/s0021-9258(18)98896-4
发表时间：
1991-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. S. Bischoff;G. Ordal
通讯作者：
D. S. Bischoff;G. Ordal
DOI：
10.1073/pnas.86.22.8703
发表时间：
1989-11-01
影响因子：
11.1
作者：
LIU, JD;PARKINSON, JS
通讯作者：
PARKINSON, JS